{"id":3417,"date":"2021-01-28T23:01:11","date_gmt":"2021-01-28T15:01:11","guid":{"rendered":"http:\/\/dh.oncolab.cn\/index.php\/2021\/01\/28\/%e5%85%8d%e7%96%ab%e5%ad%a6%e6%8b%be%e9%81%97-%e7%ac%ac5%e7%ab%a0%ef%bc%9a%e6%8a%97%e4%bd%93-%e6%8a%97%e5%8e%9f%e7%9b%b8%e4%ba%92%e4%bd%9c%e7%94%a8\/"},"modified":"2021-01-28T23:01:11","modified_gmt":"2021-01-28T15:01:11","slug":"%e5%85%8d%e7%96%ab%e5%ad%a6%e6%8b%be%e9%81%97-%e7%ac%ac5%e7%ab%a0%ef%bc%9a%e6%8a%97%e4%bd%93-%e6%8a%97%e5%8e%9f%e7%9b%b8%e4%ba%92%e4%bd%9c%e7%94%a8","status":"publish","type":"post","link":"http:\/\/dh.oncolab.cn\/index.php\/2021\/01\/28\/%e5%85%8d%e7%96%ab%e5%ad%a6%e6%8b%be%e9%81%97-%e7%ac%ac5%e7%ab%a0%ef%bc%9a%e6%8a%97%e4%bd%93-%e6%8a%97%e5%8e%9f%e7%9b%b8%e4%ba%92%e4%bd%9c%e7%94%a8\/","title":{"rendered":"\u514d\u75ab\u5b66\u62fe\u9057-\u7b2c5\u7ae0\uff1a\u6297\u4f53-\u6297\u539f\u76f8\u4e92\u4f5c\u7528"},"content":{"rendered":"
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\u5bfc \u8bfb<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u4e0a\u56fe\u8bf4\u660e\u4e86\u672c\u7ae0\u7684\u4e3b\u9898\uff0c\u5b83\u63cf\u8ff0\u4e86\u6297\u4f53\u5982\u4f55\u4e0e\u51e0\u4e4e\u6709\u65e0\u9650\u79cd\u5f62\u72b6\u7684\u6297\u539f\u76f8\u4e92\u4f5c\u7528\uff0c\u4ee5\u53ca\u8fd9\u79cd\u76f8\u4e92\u4f5c\u7528\u5982\u4f55\u4fdd\u62a4\u6211\u4eec\u514d\u53d7\u75c5\u539f\u4f53\u7684\u4fb5\u88ad\u3002\u672c\u7ae0\u8fd8\u56de\u987e\u4e86\u6297\u539f\u6216\u6297\u4f53\u5b58\u5728\u7684\u975e\u5e38\u7279\u5f02\u7684\u8bca\u65ad\u8bd5\u9a8c\u7684\u53d1\u5c55\u3002\u6297\u4f53-\u6297\u539f\u76f8\u4e92\u4f5c\u7528\u7684\u6700\u663e\u8457\u7684\u7279\u5f81\u662f\u5b83\u4eec\u7684\u7279\u5f02\u6027\u548c\u4eb2\u548c\u529b\u3002\u9664\u6b64\u4e4b\u5916\uff0c\u6297\u4f53-\u6297\u539f\u7684\u76f8\u4e92\u4f5c\u7528\u5f88\u50cf\u5176\u4ed6\u53d7\u4f53-\u914d\u4f53\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u6297\u4f53\u4e0e\u6297\u539f\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u6d89\u53ca\u7269\u7406\u5316\u5b66\u529b\uff0c\u7c7b\u4f3c\u4e8e\u9176\u4e0e\u5176\u5e95\u7269(\u6216\u7ade\u4e89\u6027\u6291\u5236\u5242)\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u6216\u53d7\u4f53(\u5982\u80f0\u5c9b\u7d20\u53d7\u4f53)\u4e0e\u914d\u4f53(\u5982\u80f0\u5c9b\u7d20)\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u8fd9\u4e9b\u529b\u6765\u6e90\u4e8e\u56db\u4e2a\u65b9\u9762\uff1a(1)\u5e26\u7535\u4fa7\u94fe\u4e4b\u95f4\u7684\u9759\u7535\u76f8\u4e92\u4f5c\u7528\uff0c(2)\u6c22\u952e\uff0c(3)\u8303\u5fb7\u534e\u529b\uff0c(4)\u758f\u6c34\u76f8\u4e92\u4f5c\u7528\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u5f53\u6297\u539f\u548c\u6297\u4f53\u4e4b\u95f4\u5b58\u5728\u5f88\u597d\u7684\u5339\u914d\u6027\u65f6\uff0c\u8fd9\u4e9b\u5178\u578b\u7684\u5f31\u7684\u3001\u975e\u5171\u4ef7\u76f8\u4e92\u4f5c\u7528\u7684\u603b\u548c\u53ef\u4ee5\u662f\u76f8\u5bf9\u8f83\u5f3a\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u5e76\u4e14\u8be5\u6297\u4f53\u88ab\u63cf\u8ff0\u4e3a\u5177\u6709\u9ad8\u4eb2\u548c\u529b\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6297\u4f53\u975e\u51e1\u7684\u7279\u5f02\u6027\u548c\u4eb2\u548c\u529b\u4f7f\u5176\u5728\u8bca\u65ad\u6d4b\u8bd5\u4e2d\u88ab\u5e7f\u6cdb\u4f7f\u7528\u3002\u672c\u7ae0\u7684\u540e\u534a\u90e8\u5206\u63cf\u8ff0\u4e86\u6297\u4f53-\u6297\u539f\u76f8\u4e92\u4f5c\u7528\u5728\u8bca\u65ad\u75be\u75c5\u4e2d\u7684\u51e0\u4e2a\u6700\u91cd\u8981\u7684\u5e94\u7528\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6297\u4f53\u7684\u6297\u539f\u7ed3\u5408\u4f4d\u70b9<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u8bb8\u591a\u5b9e\u9a8c\u65b9\u6cd5\u5df2\u7ecf\u88ab\u7528\u6765\u786e\u5b9a\u6297\u539f-\u6297\u4f53\u7ed3\u5408\u4f4d\u70b9\u7684\u7ed3\u6784\u3002\u5230\u76ee\u524d\u4e3a\u6b62\uff0c\u6700\u8be6\u7ec6\u548c\u6700\u6709\u4ef7\u503c\u7684\u4fe1\u606f\u6765\u81ea\u6297\u539f-\u6297\u4f53\u590d\u5408\u7269\u7684X\u5c04\u7ebf\u6676\u4f53\u5b66\u7814\u7a76\u3002\u901a\u8fc7\u5bf9\u51e0\u79cd\u6297\u539f-\u6297\u4f53\u590d\u5408\u7269\u7684\u4e09\u7ef4\u7ed3\u6784\u7684\u5206\u6790\uff0c\u5f97\u51fa\u7684\u4e00\u4e2a\u7ed3\u8bba\u662f\uff0c\u6297\u539f\u7ed3\u5408\u4f4d\u70b9\u7684\u5927\u5c0f\u548c\u5f62\u72b6\u53ef\u4ee5\u6709\u5f88\u5927\u7684\u4e0d\u540c\u3002\u4f8b\u5982\uff0c\u7ed3\u5408\u90e8\u4f4d\u53ef\u4ee5\u662f\u4e00\u4e2a\u957f\u800c\u6d45\u7684\u7f1d\u9699\uff0c\u4e5f\u53ef\u4ee5\u662f\u4e00\u4e2a\u66f4\u5bbd\u3001\u66f4\u5f00\u653e\u7684\u88c2\u7f1d\u578b\u7ed3\u6784(Fig 5.1<\/span>)\u3002\u5bf9\u4e8e\u5c0f\u5206\u5b50\u5316\u5408\u7269\uff0c\u6297\u4f53\u4e0a\u7ed3\u5408\u6297\u539f\u7684\u90e8\u4f4d\u7c7b\u4f3c\u4e8e\u9176\u6d3b\u6027\u90e8\u4f4d\u3002\u8bc6\u522b\u5b8c\u6574\u7684\u8f83\u5927\u86cb\u767d\u8d28\u5206\u5b50\u7684\u6297\u4f53\uff0c\u5b83\u662f\u9488\u5bf9\u8868\u4f4d\u7684\uff0c\u6297\u539f\u8868\u4f4d\u662f\u86cb\u767d\u8d28\u6297\u539f\u7684\u4e00\u90e8\u5206-\u5373\u6297\u4f53\u7684\u7ed3\u5408\u4f4d\u70b9-\u53ef\u80fd\u662f\u7a81\u51fa\u7684\u8868\u9762\uff0c\u800c\u4e0d\u662f\u88c2\u9699\u6216\u7f1d\u9699(\u89c1Fig 5.1<\/span>)\u3002\u5728\u6240\u6709\u60c5\u51b5\u4e0b\uff0c\u6297\u539f\u6b8b\u57fa\u548c\u6297\u4f53\u7ed3\u5408\u4f4d\u70b9\u6b8b\u57fa\u4e4b\u95f4\u5b58\u5728\u5316\u5b66\u4e92\u8865\u6027\u3002\u7ed3\u5408\u4f4d\u70b9\u7684\u58c1\u7531\u91cd\u94fe\u548c\u8f7b\u94fe(VH\u548cVL)\u7684\u53ef\u53d8\u533a(VH\u548cVL)\u7684\u6c28\u57fa\u9178\u6b8b\u57fa\u5f62\u6210\uff0c\u79f0\u4e3a\u9ad8\u53d8\u533a(HV\u533a\uff1b\u89c1\u7b2c6\u7ae0)\u3002\u6297\u4f53\u7279\u5f02\u6027\u6e90\u4e8e\u6297\u539f\u4e2d\u7684\u5316\u5b66\u57fa\u56e2\u4e0e\u6297\u4f53\u5206\u5b50\u6297\u539f\u7ed3\u5408\u4f4d\u70b9\u4e0a\u7684\u5316\u5b66\u57fa\u56e2\u4e4b\u95f4\u7684\u7cbe\u786e\u5206\u5b50\u4e92\u8865\u4ea7\u751f\u7684\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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Fig 5.1<\/span> \u6297\u539f\u7ed3\u5408\u4f4d\u70b9\u7684\u5927\u5c0f\u548c\u5f62\u72b6\u56e0\u5176\u7ed3\u5408\u7684\u5206\u5b50\u6216\u8868\u4f4d\u7684\u7c7b\u578b\u800c\u5f02<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u4ea4\u53c9\u53cd\u5e94\u6027<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u4e00\u79cd\u6297\u4f53\u5076\u5c14\u4f1a\u4e0e\u4e00\u79cd\u4ee5\u4e0a\u7684\u6297\u539f\u7ed3\u5408\uff0c\u8fd9\u79f0\u4e3a\u4ea4\u53c9\u53cd\u5e94\u6216\u591a\u7279\u5f02\u6027\u3002\u8be5\u6297\u4f53\u662f\u6297\u539f1\u7684\u7279\u5f02\u6027\u6297\u4f53\uff0c\u4f46\u4e0e\u53e6\u4e00\u4e2a\u5206\u5b50\u6297\u539f2\uff0c\u8db3\u4ee5\u4ea7\u751f\u7a33\u5b9a\u7684\u7ed3\u5408\u4f5c\u7528(Fig 5.2<\/span>)\u3002\u4ea4\u53c9\u53cd\u5e94\u7684\u53d1\u751f\u662f\u56e0\u4e3a\u6297\u539f\u548c\u6297\u4f53\u4e4b\u95f4\u5b58\u5728\u8db3\u591f\u6570\u91cf\u7684\u5316\u5b66\u76f8\u4e92\u4f5c\u7528\uff0c\u4ee5\u521b\u5efa\u4e00\u4e2a\u7a33\u5b9a\u7684\u7ed3\u6784\uff0c\u800c\u4e0d\u8003\u8651\u603b\u7684\u201c\u62df\u5408\u4f18\u5ea6\u201d\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u4ea4\u53c9\u53cd\u5e94\u53ef\u80fd\u4f1a\u4ea7\u751f\u4e34\u5e8a\u540e\u679c(BOX 5.1<\/span>)\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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Fig 5.2 <\/span>\u6297\u4f53\u4ea4\u53c9\u53cd\u5e94\u793a\u610f\u56fe<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6297\u4f53\u6216\u6297\u539f\u7684\u8bca\u65ad\u8bd5\u9a8c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u51e0\u79cd\u5e38\u89c4\u4f7f\u7528\u7684\u8bca\u65ad\u8bd5\u9a8c\u662f\u57fa\u4e8e\u6297\u4f53\u7684\u7279\u5f02\u6027\u548c\u9ad8\u4eb2\u548c\u529b\u3002\u672c\u7ae0\u7684\u5176\u4f59\u90e8\u5206\u63d0\u4f9b\u4e86\u5982\u4f55\u8fdb\u884c\u4e0d\u540c\u6d4b\u8bd5\u7684\u793a\u4f8b\uff0c\u5e76\u63cf\u8ff0\u4e86\u6bcf\u79cd\u6d4b\u8bd5\u7684\u4e00\u822c\u539f\u5219\uff1a\u8fd9\u4e9b\u68c0\u6d4b\u65e2\u6709\u5b9a\u6027\uff0c\u4e5f\u6709\u5b9a\u91cf\uff0c\u6709\u52a9\u4e8e\u786e\u5b9a\u6297\u539f\u6216\u6297\u4f53\u7684\u5b58\u5728\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u9176\u8054\u514d\u75ab\u5438\u9644\u8bd5\u9a8c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u9176\u8054\u514d\u75ab\u5438\u9644\u8bd5\u9a8c(ELISA)\u662f\u4e00\u79cd\u975e\u5e38\u654f\u611f\u548c\u7b80\u5355\u7684\u68c0\u6d4b\uff0c\u5b83\u4f7f\u7528\u4e0e\u6297\u4f53\u76f8\u8fde\u7684\u9176\u7684\u5171\u4ef7\u590d\u5408\u7269\u76f4\u63a5\u68c0\u6d4b\u6297\u539f\u6216\u4e0e\u6297\u4f53-\u6297\u539f\u590d\u5408\u7269\u7ed3\u5408(Fig 5.3<\/span>)\u3002\u8fd9\u9879\u5b9e\u9a8c\u4f7f\u7528\u7684\u662f\u5851\u6599\u677f\uff0c\u5c06\u76f8\u5173\u6297\u539f\u7ed1\u5b9a\u5728\u201c\u5b54\u201d\u7684\u5185\u90e8\u3002\u60a3\u8005\u8840\u6e05\u5728\u8fd9\u4e9b\u5b54\u4e2d\u5b75\u80b2\uff0c\u4efb\u4f55\u76f8\u5173\u6297\u4f53\u968f\u540e\u90fd\u4f1a\u4e0e\u6297\u539f\u7ed3\u5408\u3002\u5bf9\u542b\u6709\u65e0\u5173\u6297\u4f53\u7684\u8840\u6e05\u8fdb\u884c\u6e05\u6d17\uff0c\u4f46\u7531\u4e8e\u5176\u9ad8\u4eb2\u548c\u529b\uff0c\u4e0e\u6297\u539f\u7ed3\u5408\u7684\u6297\u4f53\u4e0d\u4f1a\u53d7\u5230\u5e72\u6270\u3002\u6700\u540e\uff0c\u52a0\u5165\u4ece\u52a8\u7269\u4e2d\u63d0\u53d6\u7684\u80fd\u4e0e\u9176\u7ed3\u5408\u7684\u7b2c\u4e8c\u79cd\u6297\u4f53\uff0c\u5c06\u5176\u4e0e\u60a3\u8005\u6297\u4f53\u7ed3\u5408\u3002\u8fd9\u79cd\u6297\u4eba\u514d\u75ab\u7403\u86cb\u767d\u5c06\u4e0e\u5b54\u4e2d\u4ecd\u7136\u5b58\u5728\u7684\u6297\u4f53\u7ed3\u5408\u3002\u6240\u9009\u7684\u9176\u80fd\u591f\u50ac\u5316\u53cd\u5e94\uff0c\u4ece\u65e0\u8272\u5e95\u7269(\u5982\u78b1\u6027\u78f7\u9178\u9176\u6216\u8fa3\u6839\u8fc7\u6c27\u5316\u7269\u9176[HRP]\uff1b\u89c1Fig 5.3<\/span>)\u751f\u6210\u6709\u8272\u4ea7\u7269\u3002\u7136\u540e\uff0c\u4e0e\u6297\u539f\u7ed3\u5408\u7684\u6297\u4f53\u7684\u91cf\u4e0e\u53ef\u4ee5\u770b\u5230\u7684\u6709\u8272\u6700\u7ec8\u4ea7\u7269\u7684\u91cf\u6210\u6b63\u6bd4\u3002BOX 5.2<\/span>\u663e\u793a\u4e86\u5982\u4f55\u4f7f\u7528ELISA\u6765\u7b5b\u67e5\u4f20\u67d3\u75c5\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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Fig 5.3<\/span> \u9176\u8054\u514d\u75ab\u5438\u9644\u8bd5\u9a8c(ELISA)<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u4fa7\u5411\u6d41\u52a8\u8bd5\u9a8c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u4fa7\u5411\u6d41\u52a8\u8bd5\u9a8c\u662f\u4e00\u79cd\u786e\u5b9a\u4f53\u6db2\u4e2d\u662f\u5426\u5b58\u5728\u86cb\u767d\u8d28\u3001\u6297\u539f\u6216\u6297\u4f53\u7684\u7b80\u5355\u6d4b\u8bd5\u3002\u5b83\u4e0eELISA\u68c0\u6d4b\u6709\u4e00\u4e9b\u76f8\u4f3c\u4e4b\u5904\uff0c\u4f46\u7ecf\u8fc7\u7cbe\u5fc3\u8bbe\u8ba1\uff0c\u4f7f\u7528\u8d77\u6765\u975e\u5e38\u7b80\u5355\uff0c\u4e0d\u9700\u8981\u4efb\u4f55\u7279\u6b8a\u8bbe\u5907\uff0c\u800c\u4e14\u76f8\u5bf9\u4fbf\u5b9c\u3002\u5e7f\u6cdb\u4f7f\u7528\u5728\u598a\u5a20\u8bd5\u9a8c\uff0c\u5b83\u786e\u8ba4\u5b55\u5987\u5c3f\u6db2\u4e2d\u662f\u5426\u5b58\u5728\u4eba\u7ed2\u6bdb\u819c\u4fc3\u6027\u817a\u6fc0\u7d20(HCG)\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u9a8c\u5b55\u68d2\u662f\u7531\u4e00\u79cd\u5177\u6709\u5438\u6536\u6027\u7684\u7eb8\u8d28\u6750\u6599\u5236\u6210\u7684\u3002\u653e\u7f6e\u5728\u8bd5\u68d2\u4e00\u7aef\u7684\u5c3f\u6837\u5c06\u901a\u8fc7\u6bdb\u7ec6\u7ba1\u4f5c\u7528\u88ab\u5438\u5f15\uff0c\u5e76\u5728\u6cbf\u8bd5\u6761\u7684\u8fc7\u7a0b\u4e2d\u9047\u5230\u4e0d\u540c\u7684\u8bd5\u5242(Fig 5.4<\/span>)\u3002\u7b2c\u4e00\u79cd\u8bd5\u5242\u662f\u4e0e\u6297hCG\u8868\u4f4d\u7684\u5f69\u8272\u5fae\u73e0\u76f8\u8fde\u7684\u5c0f\u9f20\u6297\u4f53\uff0c\u5f53\u5c3f\u6db2\u7b2c\u4e00\u6b21\u6ef4\u5728\u9a8c\u5b55\u68d2\u4e0a\u65f6\u4e0e\u4e4b\u6df7\u5408\uff0c\u5982\u679c\u6709hCG\u5b58\u5728\uff0c\u5c3f\u6db2\u5c31\u6210\u4e3ahCG-\u6297\u4f53-\u5fae\u73e0\u590d\u5408\u7269\u7684\u4e00\u90e8\u5206\u3002\u867d\u7136\u8fd9\u4e9b\u90fd\u662f\u5206\u6563\u7684\uff0c\u4f46\u8fd9\u4e9b\u590d\u5408\u4f53\u4ecd\u7136\u662f\u770b\u4e0d\u89c1\u7684\uff0c\u5e76\u7ee7\u7eed\u6cbf\u9a8c\u5b55\u68d2\u6d41\u52a8\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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<\/p>\n<\/section>\n<\/section>\n

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Fig 5.4<\/span> \u598a\u5a20\u68d2\u68c0\u6d4b\u5448\u9633\u6027\u7684\u4e8b\u4ef6\u987a\u5e8f<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u63a5\u4e0b\u6765\uff0c\u5c3f\u6db2\u6297\u4f53\u6df7\u5408\u7269\u5230\u8fbe\u4e00\u4e2a\u533a\u57df\uff0c\u5728\u8be5\u533a\u57df\uff0c\u9488\u5bf9\u4e0d\u540chCG\u8868\u4f4d\u7684\u7b2c\u4e8c\u6297\u4f53\u5df2\u7ecf\u56fa\u5b9a\u5728\u7eb8\u4e0a\u3002\u8be5\u6297\u4f53\u5c06\u7ed3\u5408\u5e76\u6355\u83b7\u4efb\u4f55hCG-\u6297\u4f53-\u73e0\u72b6\u590d\u5408\u7269\u3002\u5982\u679c\u6709\u8db3\u591f\u7684hCG\uff0c\u73e0\u7c92\u805a\u96c6\u7684\u5730\u65b9\u4f1a\u5f62\u6210\u4e00\u6761\u5f69\u8272\u7684\u7ebf\u6761\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6000\u5b55\u6d4b\u8bd5\u9700\u8981\u5305\u62ec\u4e00\u4e2a\u5bf9\u7167\uff0c\u4ee5\u8868\u660e\u6d4b\u8bd5\u60c5\u51b5\u4ee4\u4eba\u6ee1\u610f\u3002\u4f8b\u5982\uff0c\u5fc5\u987b\u786e\u4fdd\u5c3f\u6db2\u6cbf\u7740\u9a8c\u5b55\u68d2\u6d41\u52a8\uff0c\u4ee5\u53ca\u6297\u4f53\u73e0\u590d\u5408\u7269\u7684\u5b58\u5728\u3002\u5728\u8fd9\u4e2a\u4f8b\u5b50\u4e2d\uff0c\u5c3f\u6db2\u63a5\u4e0b\u6765\u4f1a\u9047\u5230\u7f8a\u4f53\u5185\u4ea7\u751f\u7684\u7b2c\u4e09\u79cd\u6297\u4f53\u7684\u533a\u57df\uff0c\u8be5\u6297\u4f53\u662f\u9488\u5bf9\u5c0f\u9f20\u514d\u75ab\u7403\u86cb\u767d\u7684\u3002\u8fd9\u5c06\u6355\u83b7\u6700\u540e\u51e0\u4e2a\u73e0\u5b50\uff0c\u5e76\u5c06\u4ea7\u751f\u4e00\u6761\u63a7\u5236\u7ebf\uff0c\u8868\u793a\u6d4b\u8bd5\u6210\u529f\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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Fig 5.5<\/span>\u663e\u793a\u4e86\u6000\u5b55\u6d4b\u8bd5\u7684\u9633\u6027\u548c\u9634\u6027\u7ed3\u679c\uff0c\u4ee5\u53ca\u672a\u901a\u8fc7\u7684\u68c0\u6d4b(\u4f8b\u5982\uff0c\u5f53\u4f7f\u7528\u7684\u5c3f\u6db2\u4e0d\u8db3\u65f6)\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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Fig 5.5<\/span> A\u3001\u53cc\u7ebf\u9633\u6027\u598a\u5a20\u8bd5\u9a8c\uff1bB\u3001\u5355\u884c\u9634\u6027\u8bd5\u9a8c\uff1bC\u3001\u6d4b\u8bd5\u5931\u8d25\uff0c\u65e0\u7ebf<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u514d\u75ab\u8367\u5149<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u514d\u75ab\u8367\u5149\u4f7f\u7528\u7684\u662f\u8367\u5149\u5316\u5408\u7269(\u8367\u5149\u7d20)\u5171\u4ef7\u8fde\u63a5\u7684\u6297\u4f53\u3002\u514d\u75ab\u5b66\u5bb6\u5e7f\u6cdb\u4f7f\u7528\u7684\u4e00\u79cd\u8367\u5149\u5316\u5408\u7269\u662f\u5f02\u786b\u6c30\u9178\u8367\u5149\u7d20(FITC)\uff0c\u5b83\u4e0e\u86cb\u767d\u8d28\u4e0a\u7684\u6e38\u79bb\u6c28\u57fa\u5076\u8054\u3002\u5f53FITC\u66b4\u9732\u5728\u7d2b\u5916\u7ebf(UV)\u4e0b\u65f6\u4f1a\u53d1\u51fa\u7eff\u8272\u5149\u3002\u5b89\u88c5\u7d2b\u5916\u7ebf\u5149\u6e90\u7684\u8367\u5149\u663e\u5fae\u955c\u88ab\u7528\u6765\u68c0\u67e5\u66b4\u9732\u5728\u8367\u5149\u6297\u4f53\u4e2d\u7684\u6837\u672c\u3002\u8fd9\u9879\u68c0\u6d4b\u5e7f\u6cdb\u7528\u4e8e\u68c0\u6d4b\u7ec6\u80de\u6216\u7ec4\u7ec7\u5207\u7247\u4e2d\u7684\u6297\u539f\u3002\u5b83\u8fd8\u7528\u4e8e\u7b5b\u9009\u7ec6\u80de\u6216\u7ec4\u7ec7\u6297\u539f\u7684\u81ea\u8eab\u6297\u4f53(\u7b2c28\u7ae0)\u3002\u8981\u4e48\u6d4b\u8bd5\u6297\u4f53\u76f4\u63a5\u8fde\u63a5\u5230\u8367\u5149\u5316\u5408\u7269(\u76f4\u63a5\u68c0\u6d4b)\uff0c\u8981\u4e48\u53ef\u4ee5\u8bc6\u522b\u6297\u4f53\u7684\u914d\u4f53\u8fde\u63a5\u5230\u8367\u5149\u5316\u5408\u7269(\u95f4\u63a5\u68c0\u6d4b\uff1bFig 5.6<\/span>)\u3002\u901a\u5e38\uff0c\u8367\u5149\u914d\u4f53\u662f\u9488\u5bf9\u68c0\u6d4b\u6297\u4f53(\u5982\u5c71\u7f8a\u6297\u4eba\u514d\u75ab\u7403\u86cb\u767d)\u6240\u7279\u6709\u7684\u7b2c\u4e8c\u79cd\u6297\u4f53\u3002BOX 5.2<\/span>\u63d0\u4f9b\u4e86\u95f4\u63a5\u514d\u75ab\u8367\u5149\u5206\u6790\u7684\u4e34\u5e8a\u5e94\u7528\u5b9e\u4f8b\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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Fig 5.6<\/span> \u4f7f\u7528\u5f02\u786b\u6c30\u9178\u8367\u5149\u7d20(FITC)\u8fdb\u884c\u76f4\u63a5(A)\u548c\u95f4\u63a5(B)\u514d\u75ab\u8367\u5149\u3002\u5176\u4ed6\u8367\u5149\u67d3\u6599\u53ef\u4ee5\u4e0e\u4e0d\u540c\u7684\u989c\u8272\u4e00\u8d77\u4f7f\u7528<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6d41\u5f0f\u7ec6\u80de\u672f<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6d41\u5f0f\u7ec6\u80de\u672f\u662f\u4e00\u79cd\u7528\u8ba1\u6570\u8868\u8fbe\u6297\u539f\u7684\u7ec6\u80de\u7684\u6280\u672f\u3002\u8fd9\u4e9b\u7ec6\u80de\u88ab\u7ec6\u80de\u8868\u9762\u6297\u539f\u7279\u5f02\u6027\u6297\u4f53\u67d3\u8272\u3002\u6297\u4f53\u88ab\u5076\u8054\u5230\u7279\u5b9a\u7684\u8367\u5149\u8bd5\u5242\uff0c\u5982FITC(\u8fd8\u6709\u5176\u4ed6\u51e0\u79cd\u4e0d\u540c\u989c\u8272\u7684\u8367\u5149\u53ef\u4f9b\u9009\u62e9)\uff0c\u7136\u540e\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u4eea\u3002\u67d3\u8272\u7ec6\u80de\u7684\u6570\u91cf\u53ef\u4ee5\u8ba1\u6570\uff0c\u4f8b\u5982CD4+T\u7ec6\u80de\u7684\u6570\u91cf(Fig 5.7<\/span>\uff1b\u53e6\u4e00\u4e2a\u4f8b\u5b50\u89c1\u7b2c35\u7ae0)\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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Fig 5.7<\/span> \u6d41\u5f0f\u7ec6\u80de\u4eea\u201c\u6563\u70b9\u56fe\u201d<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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BOX 5.1 <\/span>\u836f\u7269\u8fc7\u654f-\u514d\u75ab\u7403\u86cb\u767dE\u4ea4\u53c9\u53cd\u5e94<\/span><\/p>\n<\/section>\n<\/section>\n

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<\/svg><\/section>\n<\/section>\n
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\u4e00\u540d58\u5c81\u7684\u5987\u5973\u88ab\u8bca\u65ad\u51fa\u60a3\u6709\u80ba\u708e\uff0c\u6025\u9700\u6297\u751f\u7d20\u3002\u7ea620\u5e74\u524d\uff0c\u533b\u751f\u7ed9\u5979\u5f00\u4e86\u9752\u9709\u7d20\u6cbb\u7597\u5589\u5499\u75db\uff0c\u5728\u5979\u670d\u7528\u7b2c\u4e00\u7c92\u80f6\u56ca\u540e\u768430\u5206\u949f\u5185\uff0c\u5979\u51fa\u73b0\u5e7f\u6cdb\u7684\u76ae\u75b9\uff0c\u547c\u5438\u56f0\u96be\uff0c\u4f11\u514b\u3002\u5979\u7684\u4e3b\u6cbb\u533b\u751f\u8ba4\u4e3a\u53ef\u80fd\u662f\u9752\u9709\u7d20\u8fc7\u654f\u53d1\u4f5c\u3002\u533b\u751f\u77e5\u9053\uff0c\u9752\u9709\u7d20\u8fc7\u654f\u7684\u4eba\u4e0d\u5e94\u8be5\u518d\u63a5\u53d7\u9752\u9709\u7d20\u6cbb\u7597\uff0c\u5e76\u4e14\u5bf9\u03b2-\u5185\u9170\u80fa\u7c7b\u7684\u5176\u4ed6\u6297\u751f\u7d20\uff0c\u5982\u4e00\u4e9b\u5934\u5b62\u83cc\u7d20\u548c\u78b3\u9752\u9709\u70ef\u7c7b\u4e5f\u4f1a\u6709\u5927\u7ea65%\u523010%\u7684\u53cd\u5e94\u98ce\u9669\u3002\u4ed6\u51b3\u5b9a\u7528\u7ea2\u9709\u7d20\u6cbb\u7597\u75c5\u4eba\uff0c\u7ea2\u9709\u7d20\u7684\u5206\u5b50\u7ed3\u6784\u548c\u9752\u9709\u7d20\u975e\u5e38\u4e0d\u540c\u3002\u75c5\u4eba\u5bf9\u8fd9\u79cd\u6297\u751f\u7d20\u53cd\u5e94\u826f\u597d\uff0c\u6ca1\u6709\u526f\u4f5c\u7528\u3002<\/p>\n

\u836f\u7269\u7684\u4e0d\u826f\u514d\u75ab\u53cd\u5e94\uff0c\u7279\u522b\u662f\u6297\u751f\u7d20\uff0c\u53ef\u80fd\u662f\u4e00\u4e2a\u91cd\u5927\u7684\u533b\u5b66\u95ee\u9898\u3002\u9752\u9709\u7d20\u53ef\u4ee5\u4e0e\u81ea\u8eab\u86cb\u767d\u5f62\u6210\u534a\u6297\u539f\u8f7d\u4f53\u7ed3\u5408\u7269\uff0c\u7136\u540e\u4f5c\u4e3a\u514d\u75ab\u539f\u4ea7\u751f\u514d\u75ab\u7403\u86cb\u767dE(IgE)\u6297\u4f53\u3002\u4e0d\u5e78\u7684\u662f\uff0c\u6297\u9752\u9709\u7d20IgE\u6297\u4f53\u4e5f\u4f1a\u4e0e\u5176\u4ed6\u4e00\u4e9b\u6297\u751f\u7d20\u53d1\u751f\u4ea4\u53c9\u53cd\u5e94\u3002\u8fd9\u53ef\u80fd\u4f1a\u4f7f\u8fd9\u4e9b\u60a3\u8005\u7684\u7ec6\u83cc\u611f\u67d3\u6cbb\u7597\u590d\u6742\u5316\uff0c\u56e0\u4e3a\u4ed6\u4eec\u65e0\u6cd5\u670d\u7528\u5bf9\u6297\u611f\u67d3\u6240\u9700\u7684\u6297\u751f\u7d20\u3002<\/p>\n

\u9752\u9709\u7d20\u662f\u4e00\u79cd\u03b2-\u5185\u9170\u80fa\u7c7b\u6297\u751f\u7d20\uff0c\u4e4b\u6240\u4ee5\u8fd9\u6837\u547d\u540d\uff0c\u662f\u56e0\u4e3a\u5b83\u542b\u6709\u56db\u5143\u03b2-\u5185\u9170\u80fa\u73af\u7ed3\u6784\uff0c\u5982Fig 5.3<\/span>\u6240\u793a\u3002\u4e00\u4e9b\u6297\u9752\u9709\u7d20IgE\u6297\u4f53\u53ef\u4ee5\u4e0e\u5176\u4ed6\u7ed3\u6784\u76f8\u4f3c\u7684\u6297\u751f\u7d20\u53cd\u5e94\u3002\u5177\u4f53\u7684\u7279\u5f02\u6027\u53ef\u80fd\u4f1a\u6709\u6240\u4e0d\u540c\uff0c\u4f46\u6709\u8db3\u591f\u7684\u201c\u9002\u5408\u5ea6\u201d(\u4ea4\u53c9\u53cd\u5e94\u6027)\u53ef\u4ee5\u663e\u8457\u7ed3\u5408\u8fd9\u4e9b\u5176\u4ed6\u7684\u6297\u751f\u7d20\uff0c\u5e76\u9020\u6210\u6cbb\u7597\u95ee\u9898(Fig 5.8<\/span>)\u3002<\/p>\n<\/section>\n<\/section>\n

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Fig 5.8 <\/span>\u9752\u9709\u7d20\u53ca\u5176\u76f8\u5173\u6297\u751f\u7d20\u7684\u7ed3\u6784\u3002\u9ec4\u8272\u7684\u662f\u03b2-\u5185\u9170\u80fa\u73af<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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BOX 5.2<\/span> \u4f20\u67d3\u75c5\u7684\u7b5b\u67e5<\/p>\n<\/section>\n<\/section>\n

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\u7b5b\u67e5\u732e\u8840\u8005\u662f\u5426\u60a3\u6709\u53ef\u80fd\u901a\u8fc7\u8840\u6db2\u4f20\u64ad\u7684\u4f20\u67d3\u75c5\uff0c\u7b5b\u67e5\u7684\u5176\u4e2d\u4e00\u4e2a\u76ee\u7684\u662f\u5224\u65ad\u4ed6\u4eec\u662f\u5426\u6709\u611f\u67d3\u8fd9\u4e9b\u4f20\u67d3\u75c5\u7684\u98ce\u9669\uff0c\u5982\u679c\u6709\u90a3\u4e48\u5c31\u4f1a\u88ab\u6392\u9664\u3002\u4e00\u4e2a\u4f8b\u5b50\u662f\u7537\u6027\u4e4b\u95f4\u53d1\u751f\u6027\u884c\u4e3a\uff0c\u5728\u4e00\u4e9b\u56fd\u5bb6\u8fd9\u4e00\u7fa4\u4f53\u53ef\u80fd\u56e0\u4e3a\u611f\u67d3\u827e\u6ecb\u75c5\u6bd2\u3001\u6885\u6bd2\u6216\u75c5\u6bd2\u6027\u809d\u708e\u7684\u98ce\u9669\u8f83\u5927\u800c\u88ab\u6392\u9664\u5728\u5916\u3002\u8fd8\u4f1a\u8fdb\u884c\u8840\u6db2\u6d4b\u8bd5\uff0c\u4ee5\u6392\u9664\u611f\u67d3\u98ce\u9669\u3002\u8fd9\u4e9b\u6d4b\u8bd5\u68c0\u6d4b\u5728\u611f\u67d3\u6216\u63a5\u79cd\u75ab\u82d7\u540e\u4ea7\u751f\u7684\u9488\u5bf9\u75c5\u539f\u4f53\u7684\u6297\u4f53\uff0c\u5c31\u50cf\u4e59\u809d\u75c5\u6bd2\u4e00\u6837\u3002<\/p>\n

Fig 5.9<\/span>\u663e\u793a\u4e86\u6885\u6bd2\u7684\u9176\u8054\u514d\u75ab\u5438\u9644\u8bd5\u9a8c(ELISA)\u7b5b\u67e5\u8bd5\u9a8c\u3002ELISA\u8bd5\u677f\u5305\u542b\u4e24\u4e2a\u5bf9\u7167\u5b54\u548c94\u4e2a\u6f5c\u5728\u732e\u8840\u8005\u7684\u6837\u672c\u3002\u7b2c\u4e00\u4e2a\u5b54\u662f\u7528\u4e00\u540d\u6885\u6bd2\u60a3\u8005\u7684\u8840\u6e05\u8fdb\u884c\u9633\u6027\u5bf9\u7167\u3002\u7b2c\u4e8c\u4e2a\u5b54\u542b\u6709\u4ece\u672a\u60a3\u8fc7\u6885\u6bd2\u7684\u4eba\u7684\u8840\u6e05\uff0c\u8fd9\u662f\u4e00\u79cd\u9634\u6027\u5bf9\u7167\u3002\u8fd9\u4e24\u4e2a\u63a7\u4ef6\u5408\u5e76\u5728\u4e00\u8d77\u662f\u4e3a\u4e86\u786e\u4fdd\u6d4b\u8bd5\u66f4\u52a0\u51c6\u786e\u3002\u53e6\u4e00\u4e2a\u5b54\u663e\u793a\u4e3a\u9ec4\u8272\uff0c\u8fd9\u8868\u660e\u7ed3\u679c\u662f\u9633\u6027\u7684\u3002<\/p>\n<\/section>\n<\/section>\n

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Fig 5.9 <\/span>\u68c0\u6d4b\u6885\u6bd2\u7684\u9176\u8054\u514d\u75ab\u5438\u9644\u8bd5\u9a8c(ELISA)\u8bd5\u9a8c\u4e2d\u7528\u4e8e\u68c0\u6d4b94\u4efd\u8840\u6db2\u6837\u672c\u677f<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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BOX 5.2<\/span> \u4f20\u67d3\u75c5\u7684\u7b5b\u67e5-\u7eed<\/p>\n<\/section>\n<\/section>\n

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\u6885\u6bd2\u662f\u7531\u6885\u6bd2\u87ba\u65cb\u4f53\u7684\u7ec6\u83cc\u5f15\u8d77\u7684\u4f20\u67d3\u75c5\u3002\u5b83\u7684\u4f20\u64ad\u9014\u5f84\u6709\u6027\u4f20\u64ad\u3001\u5b50\u5bab\u5185\u4f20\u64ad\u548c\u8f93\u8840\u4f20\u64ad\u3002\u8fd9\u79cd\u7b5b\u67e5\u6d4b\u8bd5\u68c0\u6d4b\u7684\u662f\u5fc3\u78f7\u8102\u7684\u78f7\u8102\u6297\u539f\u7684\u6297\u4f53\uff0c\u88ab\u79f0\u4e3a\u6027\u75c5\u7814\u7a76\u5b9e\u9a8c\u5ba4(VDRL)\u6d4b\u8bd5\u3002\u867d\u7136\u51e0\u4e4e\u6240\u6709\u7684\u6885\u6bd2\u60a3\u8005\u90fd\u4f1a\u4ea7\u751f\u8fd9\u4e9b\u6297\u4f53\uff0c\u4f46\u5b83\u4eec\u4e5f\u4f1a\u7531\u5176\u4ed6\u7c7b\u578b\u7684\u611f\u67d3\u6216\u81ea\u8eab\u514d\u75ab\u8005\u4ea7\u751f\u7684\u3002\u56e0\u6b64\uff0cVDRL\u68c0\u6d4b\u6885\u6bd2\u662f\u654f\u611f\u7684\uff0c\u4f46\u4e0d\u662f\u975e\u5e38\u5177\u6709\u7279\u5f02\u6027\u3002<\/p>\n


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\u4e3a\u4e86\u786e\u8ba4\u6f5c\u5728\u7684\u732e\u8840\u8005\u662f\u5426\u771f\u7684\u60a3\u6709\u6885\u6bd2\uff0c\u8be5\u8840\u6e05\u88ab\u7528\u4e8e\u7279\u5b9a\u7684\u514d\u75ab\u8367\u5149\u6d4b\u8bd5\u3002\u5982\u679c\u8fd9\u4e2a\u7ed3\u679c\u4e5f\u662f\u9633\u6027\u7684\uff0c\u5c31\u8bc1\u5b9e\u8fd9\u4f4d\u6350\u8d60\u8005\u786e\u5b9e\u60a3\u6709\u6885\u6bd2\uff0c\u5979\u7684\u8840\u6db2\u4e0d\u80fd\u7528\u4e8e\u8f93\u8840\uff0c\u5979\u8fd8\u4f1a\u88ab\u8f6c\u4ecb\u6cbb\u7597\u6885\u6bd2\u3002<\/p>\n


<\/p>\n

\u6885\u6bd2\u7684\u7279\u5f02\u6027\u8840\u6e05\u5b66\u68c0\u6d4b<\/strong><\/p>\n

\u6885\u6bd2\u87ba\u65cb\u4f53\u5f15\u8d77\u6885\u6bd2\u3002\u8fd9\u79cd\u7ec6\u83cc\u662f\u4e00\u79cd\u67d4\u97e7\u7684\u87ba\u65cb\u6746(Fig 5.10<\/span>)\u3002\u4e3a\u4e86\u83b7\u5f97\u5982\u56fe\u6240\u793a\u7684\u56fe\u50cf\uff0c\u8fdb\u884c\u4e86\u8367\u5149\u6885\u6bd2\u6297\u4f53\u5438\u6536\u8bd5\u9a8c(FTA-ABS)\u3002\u8bd5\u9a8c\u8840\u6e05\u9996\u5148\u88ab\u975e\u81f4\u75c5\u6027\u6885\u6bd2\u87ba\u65cb\u4f53\u5438\u6536\uff0c\u4ee5\u53bb\u9664\u4ea4\u53c9\u53cd\u5e94\u6297\u4f53\u3002\u7136\u540e\uff0c\u5c06\u5438\u6536\u7684\u8bd5\u9a8c\u8840\u6e05\u4e0e\u6885\u6bd2\u87ba\u65cb\u4f53\u5728\u663e\u5fae\u955c\u8f7d\u73bb\u7247\u4e0a\u53cd\u5e94\uff0c\u6839\u636eFig 5.6<\/span>\u4e2d\u63cf\u8ff0\u7684\u95f4\u63a5\u514d\u75ab\u8367\u5149\u6cd5\uff0c\u5728\u8367\u5149\u663e\u5fae\u955c\u4e0b\u7528\u5f02\u786b\u6c30\u9178\u8367\u5149\u7d20(FITC)\u5076\u8054\u7684\u6297\u4eba\u514d\u75ab\u7403\u86cb\u767dG(IgG)\u6297\u4f53\u68c0\u6d4b\u4efb\u4f55\u4e0e\u6885\u6bd2\u87ba\u65cb\u4f53\u7ed3\u5408\u7684\u6297\u4f53\u3002<\/p>\n<\/section>\n<\/section>\n

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Fig 5.10 <\/span>\u8367\u5149\u6885\u6bd2\u87ba\u65cb\u4f53\u6297\u4f53\u5438\u6536\u8bd5\u9a8c\u53ef\u89c1\u6885\u6bd2\u87ba\u65cb\u4f53<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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BOX 5.3<\/span> \u4e34\u5e8a\u8bca\u65ad\u5b9e\u9a8c\u5ba4\u65b0\u6280\u672f–\u8367\u5149\u5fae\u7403\u514d\u75ab\u5206\u6790<\/p>\n<\/section>\n<\/section>\n

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\u4e00\u79cd\u88ab\u79f0\u4e3aLuminex xMAP\u6280\u672f\u7684\u65b0\u8bca\u65ad\u6d4b\u8bd5\u65b9\u6cd5\uff0c\u4f3c\u4e4e\u975e\u5e38\u6709\u4ef7\u503c\uff0c\u7279\u522b\u662f\u5728\u5c0f\u4f53\u79ef\u6837\u672c\u4e0a\u8fdb\u884c\u591a\u9879\u6d4b\u8bd5\u65f6\u3002\u8fd9\u9879\u6280\u672f\u5229\u7528\u4e86\u9176\u8054\u514d\u75ab\u5438\u9644\u8bd5\u9a8c(ELISA)\u548c\u6d41\u5f0f\u7ec6\u80de\u672f\u4e24\u4e2a\u65b9\u9762\u3002\u672c\u8d28\u4e0a\uff0c\u805a\u82ef\u4e59\u70ef\u5fae\u7403\u5185\u90e8\u7528\u4e24\u79cd\u8367\u5149\u67d3\u6599\u8fdb\u884c\u989c\u8272\u7f16\u7801\uff0c\u8fd9\u4e24\u79cd\u67d3\u6599\u53ef\u4ee5\u5728\u6fc0\u5149\u7167\u5c04\u540e\u68c0\u6d4b\u5230\u3002\u901a\u8fc7\u6df7\u5408\u4e0d\u540c\u7684\u67d3\u6599\uff0c\u6700\u591a100\u9897\u73e0\u5b50\u4e2d\u7684\u6bcf\u4e00\u9897\u90fd\u53ef\u4ee5\u88ab\u8d4b\u4e88\u4e00\u4e2a\u72ec\u7279\u7684\u8eab\u4efd(\u201c\u5149\u8c31\u4fe1\u53f7\u201d)\uff0c\u8fd9\u53ef\u4ee5\u5728\u6d41\u5f0f\u7ec6\u80de\u4eea\u4e2d\u68c0\u6d4b\u5230(\u53c2\u89c1<\/span>Fig 5.7<\/span>)\u3002\u6bcf\u9897\u73e0\u5b50\u8fd8\u53ef\u4ee5\u6d82\u4e0a\u4e0d\u540c\u7684\u5316\u5408\u7269\uff0c\u5982\u6297\u4f53\u3001\u5be1\u6838\u82f7\u9178\u548c\u9176\uff0c\u8fd9\u4e9b\u5316\u5408\u7269\u53ef\u4ee5\u4ece\u6d4b\u8bd5\u6837\u672c\u4e2d\u6536\u96c6\u5206\u5b50\u3002\u901a\u8fc7\u4f7f\u7528\u5e26\u6709\u4e0d\u540c\u989c\u8272\u8367\u5149\u62a5\u544a\u6807\u7b7e\u7684\u5939\u5fc3\u6cd5(\u5982Fig 5.11<\/span>\u6240\u793a)\uff0c\u53ef\u4ee5\u7528\u7b2c\u4e8c\u4e2a\u6fc0\u5149\u6d4b\u91cf\u5316\u5408\u7269(\u4f8b\u5982\uff0c\u809d\u708e\u75c5\u6bd2\u6297\u4f53)\u7684\u91cf\u3002\u5fae\u7403\u6eb6\u89e3\u5728\u5fae\u6ef4\u5b9a\u5b54\u6216\u8bd5\u7ba1\u4e2d\uff0c\u5e76\u4e14\u591a\u4e2a\u73e0\u5b50\u53ef\u4ee5\u5b58\u5728\u4e8e\u5355\u4e2a\u5bb9\u5668\u4e2d\u3002\u6bcf\u4e00\u9897\u73e0\u5b50\u90fd\u53ef\u4ee5\u7528\u4e0d\u540c\u7684\u53cd\u5e94\u7269\u884d\u751f\u5316\uff0c\u4e00\u7ec4100\u9897\u73e0\u5b50\u53ef\u4ee5\u5206\u6790\u591a\u8fbe100\u79cd\u4e0d\u540c\u7684\u5316\u5408\u7269\u3002\u73e0\u5b50\u5728\u6db2\u4f53\u4e2d\u88ab\u8f93\u9001\u5230\u5206\u6790\u4eea\u4e2d\uff0c\u5e76\u53d7\u5230\u6fc0\u5149\u7167\u5c04\uff0c\u5f88\u50cf\u6d41\u5f0f\u7ec6\u80de\u4eea\u4e2d\u7684\u5355\u4e2a\u7ec6\u80de(\u89c1Fig 5.7<\/span>)\u3002\u8fd9\u9879\u6280\u672f\u53ef\u4ee5\u7528\u6765\u5728\u5f88\u77ed\u7684\u65f6\u95f4\u5185\u4ece\u5c11\u91cf\u7684\u6837\u54c1\u4e2d\u8fdb\u884c\u591a\u9879\u6d4b\u8bd5\uff0c\u79f0\u4e3a\u591a\u91cd\u6d4b\u8bd5\u3002\u7ed3\u679c\u8868\u660e\uff0c\u8be5\u68c0\u6d4b\u65b9\u6cd5\u7075\u654f\u3001\u7279\u5f02\uff0c\u4e0eELISA\u7b49\u5176\u4ed6\u68c0\u6d4b\u65b9\u6cd5\u76f8\u6bd4\u5177\u6709\u4e00\u5b9a\u7684\u4f18\u52bf\u3002<\/p>\n

\u4e0b\u9762\u662f\u53ef\u4ee5\u4f7f\u7528\u8be5\u6d4b\u8bd5\u7684\u4e00\u4e2a\u793a\u4f8b\u3002\u4e00\u4e2a\u75c5\u4eba\u5728\u51fa\u56fd\u65c5\u884c\u540e\u4e0d\u4e45\u5c31\u51fa\u73b0\u4e86\u9ec4\u75b8\u3002\u75c5\u4eba\u58f0\u79f0\u5728\u65c5\u884c\u671f\u95f4\u6ca1\u6709\u9ad8\u5371\u884c\u4e3a\u53f2\uff0c\u7136\u540e\u4ed6\u88ab\u8981\u6c42\u68c0\u6d4b\u809d\u708e\u75c5\u6bd2\u6297\u4f53\u3002\u91c7\u96c6\u8840\u6db2\u6837\u672c\uff0c\u8840\u6e05\u7528\u4e8e\u68c0\u6d4b\u7532\u578b\u3001\u4e59\u578b\u548c\u4e19\u578b\u809d\u708e\u75c5\u6bd2\u6297\u4f53\u7684\u5b58\u5728\u3002\u5982\u56fe\u6240\u793a\uff0c\u60a3\u8005\u8840\u6e05\u4e2d\u542b\u6709\u4e0e\u8868\u8fbe\u7532\u578b\u809d\u708e\u6297\u539f\u7684Luminex\u5c0f\u7403\u7ed3\u5408\u7684\u6297\u4f53\uff0c\u800c\u4e0d\u662f\u4e0e\u8868\u8fbe\u4e59\u578b\u809d\u708e\u6216\u4e19\u578b\u809d\u708e\u6297\u539f\u7684\u5c0f\u7403\u7ed3\u5408\u7684\u6297\u4f53\u3002\u60a3\u8005\u63a5\u53d7\u7532\u578b\u809d\u708e\u6cbb\u7597\uff0c\u9ec4\u75b8\u6d88\u9000\u3002<\/p>\n

\u5fae\u7403\u5fae\u73e0\u68c0\u6d4b\u7684\u4e00\u4e2a\u4f18\u70b9\u662f\u80fd\u591f\u540c\u65f6\u68c0\u6d4b\u591a\u79cd\u53cd\u5e94\u7269\uff0c\u4f8b\u5982\u4e00\u7ec4\u75c5\u6bd2\u6297\u539f\u6216\u4e00\u7ec4\u7ec6\u80de\u56e0\u5b50\u3002<\/p>\n<\/section>\n<\/section>\n

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Fig 5.11 <\/span>\u57fa\u4e8e\u8367\u5149\u5fae\u7403\u7684\u809d\u708e\u75c5\u6bd2\u6297\u4f53\u514d\u75ab\u5206\u6790(Luminex xMAP\u6280\u672f)<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u672c\u6587\u7531Oncolab\u5b9e\u9a8c\u5ba4\u5fae\u4fe1\u516c\u4f17\u53f7\u7ffb\u8bd1\u6574\u7406\u81eaImmunology for Medical Students\uff0c\u4ec5\u7528\u4e8e\u5b66\u4e60\u4ea4\u6d41\u4f7f\u7528\uff0c\u5982\u9700\u8d2d\u4e70\u539f\u7248\u4e66\u7c4d\u53ef\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8d2d\u4e70\u3002<\/span>
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\u2014 THE  END \u2014<\/span><\/p>\n

\u6765\u6e90 | Immunology for Medical Students<\/span><\/section>\n
\u7ffb\u8bd1 | <\/span><\/span>\u848b\u4fca\u535a<\/span> \u5f90\u66fc\u65af \u674e\u6f84\u7199<\/span> <\/span><\/section>\n
\u7f16\u8f91\u6392\u7248 |<\/span> \u848b\u4fca\u535a<\/span><\/span><\/section>\n
\u5ba1\u6838 |  \u738b\u5764 \u5b8b\u4e50<\/span><\/section>\n

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\u2589  \u5f80\u671f\u7cbe\u5f69\u5185\u5bb9\u63a8\u8350<\/strong><\/strong><\/p>\n

\u514d\u75ab\u5b66\u62fe\u9057-\u7b2c4\u7ae0\uff1a\u6297\u539f\u548c\u6297\u4f53\u7ed3\u6784<\/a><\/span><\/p>\n

\u514d\u75ab\u5b66\u62fe\u9057-\u7b2c3\u7ae0\uff1a\u6297\u539f\u8bc6\u522b<\/a><\/span><\/p>\n

\u514d\u75ab\u5b66\u62fe\u9057-\u7b2c2\u7ae0\uff1a\u514d\u75ab\u7cfb\u7edf\u7684\u57fa\u672c\u6982\u5ff5\u548c\u7ec4\u6210<\/a><\/span>
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\u514d\u75ab\u5b66\u62fe\u9057-\u7b2c1\u7ae0\uff1a\u514d\u75ab\u7cfb\u7edf\u7b80\u4ecb<\/a><\/span><\/p>\n

GraphPad+AI\u5236\u4f5c\u53ef\u53d1\u8868\u7ea7\u522b\u7684\u67f1\u72b6\u56fe-OncoLab\u5b9e\u9a8c\u5ba4<\/span><\/a><\/p>\n

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PD-1\/PD-L1\u963b\u65ad\u5242\u6cbb\u7597\u80bf\u7624\u7684\u7814\u7a76\u8fdb\u5c55\u4e0e\u9762\u4e34\u7684\u6311\u6218<\/a><\/span><\/section>\n
Immunity\uff1a\u80bf\u7624\u514d\u75ab\u6cbb\u7597\u7684\u5341\u5927\u6311\u6218<\/span><\/a>
<\/section>\n
BD LSR Fortessa\u6d41\u5f0f-\u4e2d\u897f\u533b\u7ed3\u5408\u57fa\u7840\u7814\u7a76\u4e2d\u5fc3<\/a><\/span><\/section>\n
\u8521\u53f8LSM800\u6fc0\u5149\u5171\u805a\u7126\u663e\u5fae\u955c\u64cd\u4f5c\u89c6\u9891-\u4e2d\u897f\u533b\u7ed3\u5408\u57fa\u7840\u7814\u7a76\u4e2d\u5fc3<\/a><\/span><\/section>\n
\u5982\u4f55\u4f7f\u7528ImageJ\u8fdb\u884c\u7ec6\u80de\u8367\u5149\u5b9a\u91cf<\/span><\/a>
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\u5982\u4f55\u4f7f\u7528Scrivener\u8fdb\u884c\u79d1\u6280\u8bba\u6587\u5199\u4f5c<\/span><\/a>
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Mendeley-\u6700\u597d\u7528\u7684\u6587\u732e\u9605\u8bfb\u7ba1\u7406\u8f6f\u4ef6\uff0c\u6ca1\u6709\u4e4b\u4e00<\/span><\/a>
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\u5982\u4f55\u9009\u62e9\u4e00\u4e2a\u5408\u9002\u7684\u6742\u5fd7\u8fdb\u884c\u6295\u7a3f\uff1f<\/span><\/a>
<\/section>\n
PubMed\u4e2d\u5982\u4f55\u6309\u5f71\u54cd\u56e0\u5b50\u7b5b\u9009\u68c0\u7d22\u7ed3\u679c\u5e76\u663e\u793a\u5f71\u54cd\u56e0\u5b50\u53ca\u5206\u533a\uff1f<\/span><\/a>
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\u5982\u4f55\u4f7f\u7528EndNote\u8f6f\u4ef6\u63d2\u5165\u53c2\u8003\u6587\u732e\uff08\u96446813\u79cd\u6742\u5fd7\u53c2\u8003\u6587\u732e\u683c\u5f0f\u6587\u4ef6\uff09<\/span><\/a>
<\/section>\n
\u5982\u4f55\u6d4b\u91cfWestern Blot\u6761\u5e26\u7070\u5ea6\u503c\uff1f(GelPro\u8f6f\u4ef6\u4f7f\u7528\u65b9\u6cd5)<\/span><\/a>
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\u5b9e\u9a8c\u4e2d\u5982\u4f55\u6311\u9009\u5230\u4e00\u652f\u597d\u7528\u7684\u6297\u4f53\uff1f<\/a><\/span><\/p>\n


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\u957f\u6309\u4e0a\u65b9\u4e8c\u7ef4\u7801\u5373\u53ef\u5173\u6ce8\u672c\u53f7~<\/span><\/p>\n


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\u70b9\u4eae\u201c\u5728\u770b\u201d<\/span><\/section>\n
\u8ba9\u66f4\u591a\u4eba\u770b\u5230\uff01<\/span><\/section>\n
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\u672c\u7bc7\u6587\u7ae0\u6765\u6e90\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7:OncoLab<\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u5bfc \u8bfb \u4e0a\u56fe\u8bf4\u660e\u4e86\u672c\u7ae0\u7684\u4e3b\u9898\uff0c\u5b83\u63cf\u8ff0\u4e86\u6297\u4f53\u5982\u4f55\u4e0e\u51e0\u4e4e\u6709\u65e0\u9650\u79cd\u5f62\u72b6\u7684\u6297\u539f\u76f8\u4e92\u4f5c\u7528\uff0c\u4ee5\u53ca\u8fd9\u79cd\u76f8\u4e92\u4f5c\u7528\u5982\u4f55\u4fdd\u62a4\u6211\u4eec\u514d\u53d7\u75c5\u539f\u4f53\u7684\u4fb5\u88ad\u3002\u672c\u7ae0\u8fd8\u56de\u987e\u4e86\u6297\u539f\u6216\u6297\u4f53\u5b58\u5728\u7684\u975e\u5e38\u7279\u5f02\u7684\u8bca\u65ad\u8bd5\u9a8c\u7684\u53d1\u5c55\u3002…<\/p>\n","protected":false},"author":1,"featured_media":3402,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[58],"tags":[],"_links":{"self":[{"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/posts\/3417"}],"collection":[{"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/comments?post=3417"}],"version-history":[{"count":0,"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/posts\/3417\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/media\/3402"}],"wp:attachment":[{"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/media?parent=3417"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/categories?post=3417"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/tags?post=3417"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}