{"id":5472,"date":"2024-03-27T13:24:47","date_gmt":"2024-03-27T05:24:47","guid":{"rendered":"http:\/\/dh.oncolab.cn\/index.php\/2024\/03\/27\/nature%ef%bc%9a%e4%bc%a4%e5%ae%b3%e6%84%9f%e5%8f%97%e7%a5%9e%e7%bb%8f%e5%85%83%e5%bd%b1%e5%93%8d%e8%82%bf%e7%98%a4%e5%85%8d%e7%96%ab%e7%9b%91%e8%a7%86\/"},"modified":"2024-03-27T13:24:47","modified_gmt":"2024-03-27T05:24:47","slug":"nature%ef%bc%9a%e4%bc%a4%e5%ae%b3%e6%84%9f%e5%8f%97%e7%a5%9e%e7%bb%8f%e5%85%83%e5%bd%b1%e5%93%8d%e8%82%bf%e7%98%a4%e5%85%8d%e7%96%ab%e7%9b%91%e8%a7%86","status":"publish","type":"post","link":"http:\/\/dh.oncolab.cn\/index.php\/2024\/03\/27\/nature%ef%bc%9a%e4%bc%a4%e5%ae%b3%e6%84%9f%e5%8f%97%e7%a5%9e%e7%bb%8f%e5%85%83%e5%bd%b1%e5%93%8d%e8%82%bf%e7%98%a4%e5%85%8d%e7%96%ab%e7%9b%91%e8%a7%86\/","title":{"rendered":"Nature\uff1a\u4f24\u5bb3\u611f\u53d7\u795e\u7ecf\u5143\u5f71\u54cd\u80bf\u7624\u514d\u75ab\u76d1\u89c6"},"content":{"rendered":"
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\u524d\u671f\u7cbe\u5f69\u5185\u5bb9\uff1a<\/span><\/p>\n

Nature Cancer\uff1a\u80bf\u7624\u76f8\u5173\u9ad3\u7cfb\u7ec6\u80de\u4e0a\u7684Sirp\u03b1\u5728\u7ed3\u76f4\u80a0\u764c\u4e2d\u72ec\u7acb\u4e8e\u5176\u4e0eCD47\u76f8\u4e92\u4f5c\u7528\u6291\u5236\u6297\u80bf\u7624\u514d\u75ab<\/span><\/a><\/p>\n

Cell Metab\uff1a\u80bf\u7624\u5206\u6ccc\u7684FGF21\u901a\u8fc7\u91cd\u5851CD8+T\u7ec6\u80de\u7684\u80c6\u56fa\u9187\u4ee3\u8c22\u53d1\u6325\u514d\u75ab\u6291\u5236\u4f5c\u7528<\/span><\/a><\/p>\n

Cell\uff1aSMARCAL1\u662f\u4fc3\u8fdb\u80bf\u7624\u514d\u75ab\u9003\u9038\u7684\u5929\u7136\u514d\u75ab\u4fe1\u53f7\u548cPD-L1\u8868\u8fbe\u7684\u53cc\u91cd\u8c03\u8282\u5668<\/span><\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6742\u5fd7\u540d\u79f0<\/strong>\uff1aNature<\/span><\/p>\n

\u53d1\u8868\u65e5\u671f<\/strong>\uff1a2022.11.02<\/p>\n

DOI<\/strong>\uff1a10.1038\/s41586-022-05374-w.<\/p>\n

PubMed<\/strong>\uff1ahttps:\/\/pubmed.ncbi.nlm.nih.gov\/36323780\/<\/p>\n

\u5f71\u54cd\u56e0\u5b50<\/strong>\uff1a64.8<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u5185\u5bb9\u7b80\u4ecb<\/strong><\/p>\n<\/section>\n

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\u5b9e\u4f53\u80bf\u7624\u53d7\u5230\u81ea\u4e3b\u795e\u7ecf\u548c\u5468\u56f4\u611f\u89c9\u795e\u7ecf\u7cfb\u7edf\u4e2d\u795e\u7ecf\u7ea4\u7ef4\u7684\u652f\u914d\u3002\u76ee\u524d\u5c1a\u4e0d\u660e\u786e\u75db\u89c9\u611f\u53d7\u795e\u7ecf\u5143\uff08\u4f24\u5bb3\u611f\u53d7\u5668\uff09\u5bf9\u80bf\u7624\u65b0\u751f\u795e\u7ecf\u5185\u5316\u53ca\u5176\u5bf9\u764c\u75c7\u514d\u75ab\u76d1\u89c6\u7684\u5f71\u54cd\u3002\u672c\u7814\u7a76\u63ed\u793a\u4e86\u9ed1\u8272\u7d20\u7624\u7ec6\u80de\u4e0e\u75db\u89c9\u795e\u7ecf\u5143\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u8fd9\u4e00\u4e92\u52a8\u4fc3\u8fdb\u4e86\u795e\u7ecf\u7a81\u8d77\u7684\u751f\u957f\u3001\u589e\u52a0\u4e86\u5bf9\u6709\u5bb3\u914d\u4f53\u7684\u54cd\u5e94\u6027\u4ee5\u53ca\u795e\u7ecf\u80bd\u7684\u91ca\u653e\u3002\u7279\u522b\u5730\uff0c\u75db\u89c9\u795e\u7ecf\u5143\u4ea7\u751f\u7684\u795e\u7ecf\u80bd\u2014\u2014\u964d\u9499\u7d20\u57fa\u56e0\u76f8\u5173\u80bd\uff08CGRP\uff09\u2014\u2014\u76f4\u63a5\u5bfc\u81f4\u4e86\u7ec6\u80de\u6bd2\u6027CD8+ <\/sup>T\u7ec6\u80de\u7684\u8017\u7aed\uff0c\u51cf\u5f31\u4e86\u5b83\u4eec\u6e05\u9664\u9ed1\u8272\u7d20\u7624\u7684\u80fd\u529b\u3002TRPV1\u8c31\u7cfb\u7684\u57fa\u56e0\u5220\u9664\u3001\u75db\u89c9\u795e\u7ecf\u5143\u7684\u5c40\u90e8\u836f\u7406\u6291\u5236\u4ee5\u53caCGRP\u53d7\u4f53RAMP1\u7684\u963b\u65ad\uff0c\u90fd\u80fd\u51cf\u8f7b\u80bf\u7624\u6d78\u6da6\u7684\u6dcb\u5df4\u7ec6\u80de\u8017\u7aed\u5e76\u964d\u4f4e\u80bf\u7624\u751f\u957f\uff0c\u663e\u8457\u63d0\u9ad8\u4e86\u63a5\u79cdB16F10\u9ed1\u8272\u7d20\u7624\u7ec6\u80de\u5c0f\u9f20\u7684\u751f\u5b58\u7387\u3002\u76f8\u53cd\uff0c\u5f53\u4f7f\u7528\u5c40\u90e8\u91cd\u7ec4CGRP\u6cbb\u7597\u53bb\u9664\u75db\u89c9\u795e\u7ecf\u5143\u7684\u5c0f\u9f20\u65f6\uff0cCD8+ <\/sup>T\u7ec6\u80de\u7684\u8017\u7aed\u95ee\u9898\u518d\u6b21\u51fa\u73b0\u3002\u5728Rag1\u7f3a\u9677\u5c0f\u9f20\u4e2d\uff0c\u4e0e\u91ce\u751f\u578bCD8+ <\/sup>T\u7ec6\u80de\u76f8\u6bd4\uff0cRamp1-\/-<\/sup> CD8+ <\/sup>T\u7ec6\u80de\u5728\u5171\u79fb\u690d\u80bf\u7624\u65f6\u663e\u793a\u51fa\u66f4\u597d\u7684\u8010\u53d7\u6027\u3002\u9ed1\u8272\u7d20\u7624\u60a3\u8005\u6d3b\u68c0\u7ec4\u7ec7\u7684\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u7ed3\u679c\u8868\u660e\uff0c\u8868\u8fbeRAMP1\u7684CD8+ <\/sup>T\u7ec6\u80de\u6bd4\u4e0d\u8868\u8fbeRAMP1\u7684\u7ec6\u80de\u66f4\u6613\u8017\u7aed\uff0cRAMP1\u7684\u9ad8\u8868\u8fbe\u4e0e\u8f83\u5dee\u7684\u4e34\u5e8a\u9884\u540e\u76f8\u5173\u3002\u603b\u800c\u8a00\u4e4b\uff0c\u6211\u4eec\u7684\u7814\u7a76\u8868\u660e\uff0c\u51cf\u5c11\u80bf\u7624\u795e\u7ecf\u652f\u914d\u4e2d\u75db\u89c9\u795e\u7ecf\u5143\u91ca\u653e\u7684CGRP\uff0c\u53ef\u80fd\u662f\u901a\u8fc7\u6d88\u9664\u5176\u5bf9\u7ec6\u80de\u6bd2\u6027CD8+ <\/sup>T\u7ec6\u80de\u514d\u75ab\u8c03\u8282\u4f5c\u7528\u7684\u4e00\u79cd\u6539\u5584\u6297\u80bf\u7624\u514d\u75ab\u53cd\u5e94\u7684\u7b56\u7565\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u521b\u65b0\u6027\u53ca\u7814\u7a76\u610f\u4e49<\/strong><\/p>\n<\/section>\n

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\u8fd9\u7bc7\u6587\u7ae0\u7684\u6838\u5fc3\u521b\u65b0\u6027\u5728\u4e8e\u63ed\u793a\u4e86\u4f24\u5bb3\u611f\u53d7\u795e\u7ecf\u5143\u5982\u4f55\u901a\u8fc7\u4e0e\u9ed1\u8272\u7d20\u7624\u7ec6\u80de\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u5f71\u54cd\u80bf\u7624\u7684\u514d\u75ab\u76d1\u89c6\u673a\u5236\u3002\u7814\u7a76\u53d1\u73b0\u4f24\u5bb3\u611f\u53d7\u795e\u7ecf\u5143\u91ca\u653e\u7684\u795e\u7ecf\u80bd\u2014\u9499\u57fa\u56e0\u76f8\u5173\u80bd\uff08CGRP\uff09\u2014\u76f4\u63a5\u5bfc\u81f4\u7ec6\u80de\u6bd2\u6027CD8+ <\/sup>T\u7ec6\u80de\u529f\u80fd\u8017\u7aed\uff0c\u51cf\u5f31\u4e86\u5b83\u4eec\u6e05\u9664\u80bf\u7624\u7684\u80fd\u529b\u3002\u901a\u8fc7\u57fa\u56e0\u6d88\u878d\u3001\u836f\u7406\u5b66\u6c89\u9ed8\u548c\u53d7\u4f53\u62ee\u6297\u5b9e\u9a8c\uff0c\u7814\u7a76\u8bc1\u660e\u6291\u5236\u4f24\u5bb3\u611f\u53d7\u795e\u7ecf\u5143\u6216\u5176\u4ea7\u751f\u7684CGRP\u53ef\u4ee5\u663e\u8457\u964d\u4f4e\u80bf\u7624\u751f\u957f\uff0c\u63d0\u9ad8\u643a\u5e26\u80bf\u7624\u5c0f\u9f20\u7684\u751f\u5b58\u7387\u3002<\/p>\n

\u7814\u7a76\u7684\u610f\u4e49\u5728\u4e8e\u4e3a\u764c\u75c7\u514d\u75ab\u6cbb\u7597\u63d0\u4f9b\u4e86\u65b0\u7684\u89c6\u89d2\u548c\u6f5c\u5728\u7b56\u7565\u3002\u901a\u8fc7\u9488\u5bf9\u4f24\u5bb3\u611f\u53d7\u795e\u7ecf\u5143\u53ca\u5176\u4ea7\u751f\u7684CGRP\uff0c\u53ef\u80fd\u5f00\u53d1\u51fa\u65b0\u7684\u6cbb\u7597\u65b9\u6cd5\uff0c\u7528\u4ee5\u589e\u5f3a\u7ec6\u80de\u6bd2\u6027T\u7ec6\u80de\u7684\u529f\u80fd\uff0c\u63d0\u9ad8\u80bf\u7624\u7684\u514d\u75ab\u6e05\u9664\u80fd\u529b\u3002\u6b64\u5916\uff0c\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u63ed\u793a\u4e86RAMP1\u8868\u8fbe\u4e0eCD8+ <\/sup>T\u7ec6\u80de\u8017\u7aed\u53ca\u60a3\u8005\u9884\u540e\u4e4b\u95f4\u7684\u76f8\u5173\u6027\uff0c\u4e3a\u4e34\u5e8a\u9884\u6d4b\u548c\u6cbb\u7597\u63d0\u4f9b\u4e86\u65b0\u7684\u5206\u5b50\u6807\u5fd7\u7269\u3002\u603b\u4f53\u800c\u8a00\uff0c\u8fd9\u9879\u7814\u7a76\u4e0d\u4ec5\u589e\u8fdb\u4e86\u6211\u4eec\u5bf9\u80bf\u7624\u5fae\u73af\u5883\u4e2d\u795e\u7ecf\u514d\u75ab\u76f8\u4e92\u4f5c\u7528\u7684\u7406\u89e3\uff0c\u4e5f\u4e3a\u764c\u75c7\u6cbb\u7597\u63d0\u4f9b\u4e86\u65b0\u7684\u76ee\u6807\u548c\u7b56\u7565\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u56fe 1 | \u9ed1\u8272\u7d20\u7624\u7ec6\u80de\u4f7f\u4f24\u5bb3\u611f\u53d7\u795e\u7ecf\u5143\u654f\u611f\u5316<\/strong><\/p>\n

a\u3001\u4f24\u5bb3\u611f\u53d7\u5668\uff08Nav<\/sub>1.8cre<\/sup>::tdTomatofl\/WT<\/sup>\uff1b\u6d0b\u7ea2\u8272\uff09\u62a5\u544a\u5c0f\u9f20\u7684\u540e\u722a\u63a5\u79cd\u4e86B16F10-eGFP\u764c\u7ec6\u80de\uff08\u76ae\u5185\u6ce8\u5c04\uff0c2\u00d7105<\/sup>\u4e2a\u7ec6\u80de\uff1b\u7eff\u8272\uff09\u3002\u663e\u793aB16F10-eGFP\u63a5\u79cd\u5c0f\u9f20\u76ae\u80a4\u4e2d\u4e0eNaV<\/sub>1.8+<\/sup>\u795e\u7ecf\u7ea4\u7ef4\uff08\u6d0b\u7ea2\u8272\uff09\u6709\u795e\u7ecf\u8fde\u63a5\u7684\u4ee3\u8868\u6027\u56fe\u50cf\uff0c\u89c2\u5bdf\u65f6\u95f4\u4e3a22\u5929\u3002\u6807\u5c3a\uff0c200\u03bcm\u3002 <\/p>\n

b\u3001\u5728\u5171\u57f9\u517b\u4e2d\uff0cB16F0\u6216B16F10\u7ec6\u80de\u4f7f\u4f24\u5bb3\u611f\u53d7\u5668\u5bf9\u8fa3\u6912\u7d20\uff08100 nM\uff09\u3001\u5f02\u786b\u6c30\u9178\u70ef\u4e19\u916f\uff08AITC\uff0c100 \u03bcM\uff09\u548cATP\uff081 \u03bcM\uff09\u7684\u53cd\u5e94\u654f\u611f\u5316\uff0c\u901a\u8fc7\u9499\u79bb\u5b50\u901a\u91cf\u6d4b\u91cf\u3002\u4f4e\u6d53\u5ea6\u7684\u914d\u4f53\u5728\u5bf9\u7167\u795e\u7ecf\u5143\u4e2d\u5f15\u8d77\u6700\u5c0f\u7684\u53cd\u5e94\uff0c\u800cB16F10\u7ec6\u80de\u5bf9ATP\u7684\u654f\u611f\u6027\u8f83\u5c0f\u3002 <\/p>\n

c\u3001\u80cc\u6839\u795e\u7ecf\u8282\uff08DRG\uff09\u795e\u7ecf\u5143\u4e0eB16F10\u7ec6\u80de\u5171\u57f9\u517b\uff0896\u5c0f\u65f6\uff09\uff0c\u91ca\u653e\u7269\u8d28P\uff08SP\uff09\u3001\u8840\u7ba1\u6d3b\u6027\u80a0\u80bd\uff08VIP\uff09\u548cCGRP\u3002\u5355\u72ec\u7684B16F10\u7ec6\u80de\u4e0d\u91ca\u653e\u795e\u7ecf\u80bd\u3002\u7528KCl\uff0840 mM\uff1b30\u5206\u949f\uff09\u523a\u6fc0\u57f9\u517b\u7684\u795e\u7ecf\u5143\u53ef\u663e\u8457\u91ca\u653e\u795e\u7ecf\u80bd\u3002 <\/p>\n

d\u3001e\u3001\u539f\u59cbDRG\u795e\u7ecf\u5143\uff08Trpv1cre<\/sup>::-CheRiff-eGFPfl\/WT<\/sup>\uff09\u5355\u72ec\u57f9\u517b\u6216\u4e0eB16F10-mCherry-OVA\u7ec6\u80de\u7ec4\u5408\u57f9\u517b\u300248\u5c0f\u65f6\u540e\uff0c\u6536\u96c6\u7ec6\u80de\uff0c\u8fdb\u884cFACS\u7eaf\u5316\u548cRNA\u6d4b\u5e8f\u3002\u901a\u8fc7\u5bf9\u6392\u5e8f\u7684\u795e\u7ecf\u5143\u7684DEG\u8fdb\u884c\u5c42\u6b21\u805a\u7c7b\uff0c\u663e\u793a\u4e86\u5728\u66b4\u9732\u4e8e\u764c\u7ec6\u80de\u7684TRPV1+<\/sup>\u795e\u7ecf\u5143\u4e2d\u5bcc\u96c6\u7684\u4e0d\u540c\u8f6c\u5f55\u672c\u7ec4\uff08d\uff09\uff0c\u5305\u62ecCalca\uff08\u7f16\u7801CGRP\u7684\u57fa\u56e0\uff1be\uff09\u3002 <\/p>\n

\u6570\u636e\u663e\u793a\u4e3a\u4ee3\u8868\u6027\u56fe\u50cf\uff08a\uff09\uff0c\u4e3a\u76d2\u987b\u56fe\uff08\u4ece\u6700\u5c0f\u5230\u6700\u5927\u503c\uff1b\u76d2\u5b50\u4ece\u7b2c25\u5230\u7b2c75\u767e\u5206\u4f4d\u6570\uff0c\u4e2d\u95f4\u7ebf\u8868\u793a\u4e2d\u4f4d\u6570\uff09\uff0c\u7ed9\u51fa\u4e86\u4e2a\u4f53\u6570\u636e\u70b9\uff08b\u3001c\uff09\uff0c\u4e3a\u663e\u793a\u5f52\u4e00\u5316\u57fa\u56e0\u8868\u8fbe\u7684\u70ed\u56fe\uff08log2\uff080.01+\u6bcf\u767e\u4e07\u8bfb\u6570\uff08TPM\uff09\uff09-\u5747\u503c\uff08d\uff09\uff09\u6216\u5177\u6709\u4e2d\u4f4d\u6570\u7684\u6563\u70b9\u56fe\uff08e\uff09\u3002<\/p>\n

\u5b9e\u9a8c\u72ec\u7acb\u91cd\u590d\u4e86\u4e24\u6b21\uff08a\uff09\u6216\u4e09\u6b21\uff08b\u3001c\uff09\uff0c\u7ed3\u679c\u76f8\u4f3c\u3002RNA\u6d4b\u5e8f\u5b9e\u9a8c\u6ca1\u6709\u91cd\u590d\uff08d\u3001e\uff09\u3002<\/p>\n

n\u5982\u4e0b\uff1aa\uff1an = 4\uff1bb\uff1a\u795e\u7ecf\u5143\uff08\u6765\u81ea10\u53ea\u5c0f\u9f20\u768429\u4e2a\u795e\u7ecf\u5143\uff09\u3001B16F10\uff08\u6765\u81ea10\u4e2a\u57f9\u517b\u76bf\u768416\u4e2a\u7ec6\u80de\uff09\u3001\u795e\u7ecf\u5143 + B16F0\uff08\u6765\u81ea12\u53ea\u5c0f\u9f20\u7684387\u4e2a\u795e\u7ecf\u5143\uff09\u3001\u795e\u7ecf\u5143 + B16F10\uff08\u6765\u81ea12\u53ea\u5c0f\u9f20\u7684409\u4e2a\u795e\u7ecf\u5143\uff09\uff1bc\uff1a\u795e\u7ecf\u5143\uff08n = 12\uff09\u3001\u795e\u7ecf\u5143 + B16F10\uff08n = 12\uff09\u3001\u795e\u7ecf\u5143 + KCl\uff08n = 12\uff09\u3001B16F10\uff08n = 3\uff09\uff1bd\u3001e\uff1a\u6bcf\u7ec4n = 4\u3002<\/p>\n

P\u503c\u7531\u4e00\u5143\u65b9\u5dee\u5206\u6790\u540e\u8fdb\u884cBonferroni\uff08b\u3001c\uff09\u6216\u53cc\u4fa7\u672a\u914d\u5bf9\u7684Student’s t\u68c0\u9a8c\uff08e\uff09\u786e\u5b9a\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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<\/section>\n<\/section>\n
\n

\u56fe 2 | \u764c\u7ec6\u80de\u5206\u6ccc\u7684SLPI\u9a71\u52a8\u4f24\u5bb3\u611f\u53d7\u5668\u795e\u7ecf\u5143\u91ca\u653eCGRP<\/strong><\/p>\n

a-c, \u672a\u7ecf\u5904\u7406\u7684\u80cc\u6839\u795e\u7ecf\u8282\uff08DRG\uff09\u795e\u7ecf\u5143\uff08Trpv1cre<\/sup>::-CheRiff-eGFPfl\/WT<\/sup>\uff09\u3001B16F10-mCherry-OVA\u7ec6\u80de\u548cOVA\u7279\u5f02\u6027\u7ec6\u80de\u6bd2\u6027CD8+ <\/sup>T\u7ec6\u80de\u5355\u72ec\u6216\u7ec4\u5408\u57f9\u517b\u300248\u5c0f\u65f6\u540e\uff0c\u6536\u96c6\u7ec6\u80de\uff0c\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u6392\u5e8f\u7eaf\u5316\u5e76\u8fdb\u884cRNA\u6d4b\u5e8f\u3002<\/p>\n

a, \u5206\u9009\u795e\u7ecf\u5143\u5206\u5b50\u8f6e\u5ed3\u7684\u5c42\u6b21\u805a\u7c7b\u63cf\u7ed8\u4e86\u6bcf\u4e2a\u7ec4\u4e2d\u5bcc\u96c6\u7684\u4e0d\u540c\u8f6c\u5f55\u672c\u7fa4\u7ec4\u3002<\/p>\n

b, \u8ba1\u7b97\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\uff08DEGs\uff09\uff0c\u53d1\u73b0\u5f53\u4e0eOVA\u7279\u5f02\u6027\u7ec6\u80de\u6bd2\u6027CD8+ <\/sup>T\u7ec6\u80de\u3001DRG\u795e\u7ecf\u5143\u6216\u4e24\u79cd\u7ec6\u80de\u5171\u57f9\u517b\u65f6\uff0c\u764c\u7ec6\u80de\u4e2dSlpi\u8fc7\u8868\u8fbe\u3002<\/p>\n

c, \u5f53B16F10-mCherry-OVA\u7ec6\u80de\u4e0e\u672a\u7ecf\u5904\u7406\u7684DRG\u795e\u7ecf\u5143\u548cOVA\u7279\u5f02\u6027\u7ec6\u80de\u6bd2\u6027CD8+ <\/sup>T\u7ec6\u80de\u5171\u57f9\u517b\uff0824\u5c0f\u65f6\u621648\u5c0f\u65f6\uff09\u65f6\uff0c\u4f1a\u5206\u6cccSLPI\uff0c48\u5c0f\u65f6\u540e\u6548\u679c\u6700\u5927\u3002<\/p>\n

d-f, \u4f7f\u7528\u9499\u663e\u5fae\u955c\u6280\u672f\u53d1\u73b0\uff0cSLPI\uff0810 pg ml\u22121<\/sup> \u2013 10 ng ml\u22121<\/sup>\uff09\u6fc0\u6d3b\u4e86\u7ea620%\u7684\u57f9\u517b\u672a\u7ecf\u5904\u7406DRG\u795e\u7ecf\u5143\uff08d,e\uff09\u3002\u7528SLPI\uff083\u5c0f\u65f6\uff09\u6fc0\u6d3b\u7684\u57f9\u517b\u795e\u7ecf\u5143\u4e5f\u4f1a\u663e\u8457\u91ca\u653eCGRP\uff08f\uff09\u3002<\/p>\n

\u6570\u636e\u4ee5\u70ed\u56fe\u663e\u793a\u6807\u51c6\u5316\u57fa\u56e0\u8868\u8fbe\uff08log2<\/sub>\uff081 + TPM\uff09\u2212 \u5e73\u5747\u503c\uff08a\uff09\uff0c\u7bb1\u5f62\u56fe\uff08\u5b9a\u4e49\u89c1\u56fe 1b,c\uff09\uff08b\uff09\u6216\u5e73\u5747\u503c \u00b1 \u6807\u51c6\u8bef\uff08c\u2013f\uff09\u663e\u793a\u3002<\/p>\n

\u6837\u672c\u6570\u91cf\u5982\u4e0b\uff1aa,b: \u6bcf\u7ec4n = 2\u20134\uff1bc: \u6240\u6709\u7ec4n = 3\uff0c\u9664\u4e86CD8+ <\/sup>T\u7ec6\u80de\uff08n = 8\uff09\uff1bd: n = 17\uff1be: \u6bcf\u7ec4n = 8\uff1bf: 0 ng ml\u22121<\/sup>\uff08n = 4\uff09\uff0c0.1 ng ml\u22121<\/sup>\uff08n = 5\uff09\uff0c1 ng ml\u22121<\/sup>\uff08n = 5\uff09\uff0c5 ng ml\u22121<\/sup>\uff08n = 4\uff09\u3002<\/p>\n

c-f\u4e2d\u7684\u5b9e\u9a8c\u72ec\u7acb\u91cd\u590d\u4e09\u6b21\uff0c\u7ed3\u679c\u76f8\u4f3c\u3002\u6d4b\u5e8f\u5b9e\u9a8c\u672a\u91cd\u590d\uff08a,b\uff09\u3002<\/p>\n

P\u503c\u901a\u8fc7\u5355\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u914d\u5408\u4e8b\u540eBonferroni\u6821\u6b63\uff08b,e,f\uff09\u6216\u53cc\u4fa7\u672a\u914d\u5bf9\u5b66\u751ft\u68c0\u9a8c\u786e\u5b9a\uff08c\uff09\u3002*P \u2264 0.05, **P \u2264 0.01, ***P \u2264 0.001\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\n

\u56fe 3 | \u4f24\u5bb3\u611f\u53d7\u5668\u7684\u9057\u4f20\u6d88\u9664\u4fdd\u62a4\u6297\u80bf\u7624\u514d\u75ab\u529b<\/strong><\/p>\n

a, \u5411\u91ce\u751f\u578b\u5c0f\u9f20\u7684\u5de6\u540e\u722a\u5185\u4fa7\u6ce8\u5c04\u6b63\u4f4d\u70b9B16F10-mCherry-OVA\u7ec6\u80de\uff082 \u00d7 105<\/sup>\u4e2a\u7ec6\u80de\uff0ci.d.\uff09\u3002\u6839\u636e\u80bf\u7624\u63a5\u79cd\u540e\u7b2c13\u5929\u7684\u6d4b\u91cf\uff0c\u80bf\u7624\u5185CD8+ <\/sup>T\u7ec6\u80de\u7684\u8017\u7aed\u4e0e\u70ed\u8d85\u654f\u611f\u6027\u6b63\u76f8\u5173\uff08R2<\/sup> = 0.55, P \u2264 0.0001\uff09\u3002\u70ed\u75db\u8d85\u654f\u53cd\u6620\u4e86\u540c\u4fa7\u722a\uff08\u63a5\u79cd\u80bf\u7624\u7684\uff09\u4e0e\u5bf9\u4fa7\u722a\u7684\u64a4\u56de\u5ef6\u8fdf\u671f\u6bd4\u503c\u3002<\/p>\n

b, \u5c06\u6b63\u4f4d\u70b9B16F10-mCherry-OVA\uff085 \u00d7 105<\/sup>\u4e2a\u7ec6\u80de\uff0ci.d.\uff09\u63a5\u79cd\u5230\u4fdd\u7559\u6709\u611f\u89c9\u795e\u7ecf\u5143\uff08Trpv1WT<\/sup>::DTAfl\/WT<\/sup>\uff09\u6216\u7ecf\u6d88\u9664\u7684\uff08Trpv1cre<\/sup>::DTAfl\/WT<\/sup>\uff09\u516b\u5468\u5927\u96c4\u6027\u548c\u96cc\u6027\u5c0f\u9f20\u7684\u80c1\u4fa7\u3002\u5728\u63a5\u79cd\u540e22\u5929\u5185\u6d4b\u91cf\uff0c\u4f24\u5bb3\u611f\u53d7\u5668\u6d88\u9664\u7684\u5c0f\u9f20\u4e2d\u4f4d\u751f\u5b58\u65f6\u95f4\u589e\u52a0\u4e86\u7ea6250%\u3002<\/p>\n

c-f, \u80bf\u7624\u63a5\u79cd\u540e16\u5929\uff0c\u611f\u89c9\u795e\u7ecf\u5143\u6d88\u9664\u7684\u5c0f\u9f20\u663e\u793a\u51fa\u51cf\u5c11\u7684\u80bf\u7624\u751f\u957f\uff08c\uff09\u548c\u589e\u52a0\u7684\u80bf\u7624\u5185IFN\u03b3+<\/sup> CD8+ <\/sup>T\u7ec6\u80de\u6d78\u6da6\uff08d\uff09\uff0c\u540c\u65f6PD-1+ <\/sup>LAG3+ <\/sup>TIM3+<\/sup> CD8+ <\/sup>T\u7ec6\u80de\u7684\u6bd4\u4f8b\u964d\u4f4e\uff08e\uff09\u3002\u5728\u4f24\u5bb3\u611f\u53d7\u5668\u6d88\u9664\u7684\u5c0f\u9f20\u4e2d\uff0cB16F10-mCherry-OVA\uff085 \u00d7 105<\/sup>\u4e2a\u7ec6\u80de\uff0ci.d.\uff09\u80bf\u7624\u4f53\u79ef\u7684\u51cf\u5c11\u5728\u5168\u8eab\u6d88\u8017CD8+ <\/sup>T\u7ec6\u80de\u7684\u5c0f\u9f20\u4e2d\u672a\u89c1\uff08f\uff1b\u8bc4\u4f30\u81f3\u7b2c14\u5929\uff1b\u6297CD8\uff0c\u6bcf\u53ea\u5c0f\u9f20200\u5fae\u514b\uff0c\u8179\u8154\u6ce8\u5c04\uff0c\u6bcf3\u5929\u4e00\u6b21\uff09\u3002<\/p>\n

g,h, \u4e3a\u5316\u5b66\u6d88\u9664\u5176\u4f24\u5bb3\u611f\u53d7\u5668\u795e\u7ecf\u5143\uff0cRag1\u2212\/\u2212<\/sup>\u5c0f\u9f20\u88ab\u6ce8\u5c04RTX\u300228\u5929\u540e\uff0c\u5c0f\u9f20\u88ab\u63a5\u79cdB16F10-mCherry-OVA\uff085 \u00d7 105<\/sup>\u4e2a\u7ec6\u80de\uff0ci.d.\uff09\u3002\u63a5\u53d7RTX\u6ce8\u5c04\u4e14\u88ab\u52a8\u8f6c\u79fbOVA\u7279\u5f02\u6027CD8+ <\/sup>T\u7ec6\u80de\uff08i.v.\uff0c1 \u00d7 106<\/sup>\u7ec6\u80de\uff0c\u5f53\u80bf\u7624\u8fbe\u5230\u7ea6500 mm3<\/sup>\u65f6\uff09\u7684\u5c0f\u9f20\u663e\u793a\u51fa\u6bd4\u66b4\u9732\u4e8e\u8f7d\u4f53\u7684Rag1\u2212\/\u2212<\/sup>\u5c0f\u9f20\u66f4\u5c11\u7684\u80bf\u7624\u751f\u957f\uff08g\uff1b\u8bc4\u4f30\u81f3\u7b2c19\u5929\uff09\u548c\u8017\u7aed\uff08h\uff09\u3002<\/p>\n

\u6570\u636e\u4ee5\u7ebf\u6027\u56de\u5f52\u5206\u6790 \u00b1 s.e.\uff08a\uff09\u3001Mantel\u2013Cox\u56de\u5f52\uff08b\uff09\u3001\u5e73\u5747\u503c \u00b1 s.e.m.\uff08c,f,g\uff09\u6216\u7bb1\u5f62\u56fe\uff08\u5982\u56fe 1b,c\u5b9a\u4e49\uff09\uff0c\u5e76\u7ed9\u51fa\u5355\u72ec\u6570\u636e\u70b9\uff08d,e,h\uff09\u663e\u793a\u3002<\/p>\n

\u6837\u672c\u6570\u91cf\u5982\u4e0b\uff1aa: n = 60\uff1bb: \u5b8c\u6574\uff08n = 62\uff09\uff0c\u6d88\u9664\uff08n = 73\uff09\uff1bc: \u5b8c\u6574\uff08n = 20\uff09\uff0c\u6d88\u9664\uff08n = 25\uff09\uff1bd: \u5b8c\u6574\uff08n = 24\uff09\uff0c\u6d88\u9664\uff08n = 23\uff09\uff1be: \u5b8c\u6574\uff08n = 23\uff09\uff0c\u6d88\u9664\uff08n = 26\uff09\uff1bf: \u5b8c\u6574 + \u6297CD8\uff08n = 10\uff09\uff0c\u6d88\u9664 + \u6297CD8\uff08n = 8\uff09\uff1bg: \u8f7d\u4f53\uff08n = 12\uff09\uff0cRTX\uff08n = 10\uff09\uff1bh: \u8f7d\u4f53\uff08n = 11\uff09\uff0cRTX\uff08n = 10\uff09\u3002<\/p>\n

\u5b9e\u9a8c\u5206\u522b\u91cd\u590d\u4e24\u6b21\uff08a,f\u2013h\uff09\u6216\u516d\u6b21\uff08b\u2013e\uff09\u5f97\u5230\u76f8\u4f3c\u7ed3\u679c\u3002P\u503c\u901a\u8fc7\u7b80\u5355\u7ebf\u6027\u56de\u5f52\u5206\u6790\uff08a\uff09\u3001Mantel\u2013Cox\u56de\u5f52\uff08b\uff09\u3001\u53cc\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u914d\u5408\u4e8b\u540eBonferroni\u6821\u6b63\uff08c,f,g\uff09\u6216\u53cc\u4fa7\u672a\u914d\u5bf9\u5b66\u751ft\u68c0\u9a8c\uff08d,e,h\uff09\u786e\u5b9a\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u56fe 4 | CGRP\u8c03\u8282CD8+ T\u7ec6\u80de\u7684\u6d3b\u5316<\/strong><\/p>\n

a,b, \u6765\u81ea\u91ce\u751f\u578b\uff08a\uff09\u3001Ramp1\u2212\/\u2212<\/sup>\uff08a\uff09\u6216\u672a\u7ecf\u5904\u7406\u7684OT-I\uff08b\uff09\u5c0f\u9f20\u7684\u813e\u7ec6\u80deCD8+ <\/sup>T\u7ec6\u80de\u5728Tc1\u523a\u6fc0\u6761\u4ef6\u4e0b\uff08\u901a\u8fc7CD3\u548cCD28\u3001IL-12\u548c\u6297IL4\u5916\u6e90\u6027\u6fc0\u6d3b\uff09\u57f9\u517b48\u5c0f\u65f6\uff0c\u4ee5\u4ea7\u751f\u7ec6\u80de\u6bd2\u6027CD8+ <\/sup>T\u7ec6\u80de\u3002\u5728IL-2\uff0810 ng ml\u22121<\/sup>\uff09\u5b58\u5728\u4e0b\uff0c\u7528CGRP\uff08100 nM\uff1b\u6bcf\u4e24\u5929\u6311\u6218\u4e00\u6b21\uff09\u523a\u6fc0\u7ec6\u80de96\u5c0f\u65f6\u3002\u91ce\u751f\u578b\u7ec6\u80de\u6bd2\u6027CD8+ <\/sup>T\u7ec6\u80de\u663e\u793aPD-1+ <\/sup>LAG3+ <\/sup>TIM3+<\/sup>\u7ec6\u80de\u6bd4\u4f8b\u589e\u52a0\uff1b\u5f53\u5904\u7406\u6765\u81eaRamp1\u2212\/\u2212<\/sup>\u5c0f\u9f20\u7684\u7ec6\u80de\u6bd2\u6027CD8+ <\/sup>T\u7ec6\u80de\u65f6\uff0c\u8fd9\u79cd\u6548\u5e94\u4e0d\u5b58\u5728\uff08a\uff09\u3002\u5728\u5171\u57f9\u517b\u4e2d\uff0848\u5c0f\u65f6\uff09\uff0cCGRP\uff08100 nM\uff1b\u6bcf\u5929\u4e00\u6b21\uff09\u8fd8\u964d\u4f4e\u4e86OT-I\u7ec6\u80de\u6bd2\u6027CD8+ <\/sup>T\u7ec6\u80de\uff084 \u00d7 105<\/sup>\u4e2a\u7ec6\u80de\uff09\u6e05\u9664B16F10-mCherry-OVA\u764c\u7ec6\u80de\u7684\u80fd\u529b\uff08b\uff09\u3002<\/p>\n

c, \u5c06\u6b63\u4f4d\u70b9B16F10-mCherry-OVA\u7ec6\u80de\uff085 \u00d7 105<\/sup>\u4e2a\u7ec6\u80de\uff0ci.d.\uff09\u63a5\u79cd\u5165\u516b\u5468\u5927\u7684\u96cc\u6027\u5c0f\u9f20\uff0c\u8fd9\u4e9b\u5c0f\u9f20\u7684\u611f\u89c9\u795e\u7ecf\u5143\u4fdd\u6301\u5b8c\u6574\u6216\u88ab\u6d88\u9664\u3002\u5728\u4f24\u5bb3\u611f\u53d7\u5668\u88ab\u6d88\u9664\u7684\u5c0f\u9f20\u4e2d\uff0c\u80bf\u7624\u5468\u56f4\u6ce8\u5c04\u91cd\u7ec4CGRP\uff08100 nM\uff0ci.d.\uff0c\u6bcf\u5929\u4e00\u6b21\uff09\u53ef\u4ee5\u6062\u590dB16F10\u7684\u751f\u957f\uff08\u8bc4\u4f30\u81f3\u7b2c12\u5929\uff09\u3002<\/p>\n

d,e, \u5c06\u6b63\u4f4d\u70b9B16F10-mCherry-OVA\u7ec6\u80de\uff085 \u00d7 105<\/sup>\u4e2a\u7ec6\u80de\uff0ci.d.\uff09\u63a5\u79cd\u5165\u516b\u5468\u5927\u7684\u96c4\u6027\u548c\u96cc\u6027\u5c0f\u9f20\u3002\u4ece\u63a5\u79cd\u540e\u7b2c\u4e00\u5929\u5f00\u59cb\uff08\u5b9a\u4e49\u4e3a\u9884\u9632\u6027\uff09\uff0c\u7cfb\u7edf\u6027\u7ed9\u4e88RAMP1\u62ee\u6297\u5242BIBN4096\uff085 mg kg\u22121<\/sup>\uff09\uff0c\u6bcf\u4e24\u5929\u4e00\u6b21\uff08i.p.\uff09\u3002\u5728\u53e6\u4e00\u7ec4\u5c0f\u9f20\u4e2d\uff0c\u5f53\u80bf\u7624\u4f53\u79ef\u8fbe\u5230\u7ea6200 mm3<\/sup>\u65f6\uff0c\u5f00\u59cbBIBN4096\uff085 mg kg\u22121<\/sup>\uff0ci.p.\uff0c\u6bcf\u4e24\u5929\u4e00\u6b21\uff09\u6ce8\u5c04\uff08\u5b9a\u4e49\u4e3a\u6cbb\u7597\u6027\uff09\u3002\u9884\u9632\u6027\u6216\u6cbb\u7597\u6027BIBN4096\u6cbb\u7597\u51cf\u5c11\u4e86\u80bf\u7624\u751f\u957f\uff08d\uff09\u5e76\u964d\u4f4e\u4e86\u80bf\u7624\u5185PD-1+ <\/sup>LAG3+ <\/sup>TIM3+ <\/sup>CD8+ <\/sup>T\u7ec6\u80de\u7684\u6bd4\u4f8b\uff08e\uff1b\u8bc4\u4f30\u81f3\u7b2c13\u5929\uff09\u3002<\/p>\n

\u6570\u636e\u4ee5\u7bb1\u5f62\u56fe\uff08\u5982\u56fe 1b, c\u5b9a\u4e49\uff09\uff0c\u5e76\u7ed9\u51fa\u5355\u72ec\u6570\u636e\u70b9\uff08a,b,e\uff09\uff0c\u6216\u4ee5\u5e73\u5747\u503c \u00b1 s.e.m.\uff08c,d\uff09\u663e\u793a\u3002<\/p>\n

\u6837\u672c\u6570\u91cf\u5982\u4e0b\uff1aa: Ramp1WT<\/sup> CD8 + \u6eb6\u5242\u5bf9\u7167\uff08n = 9\uff09\uff0cRamp1WT<\/sup> CD8 + CGRP\uff08n = 10\uff09\uff0cRamp1\u2212\/\u2212<\/sup> CD8 + \u6eb6\u5242\u5bf9\u7167\uff08n = 10\uff09\uff0cRamp1\u2212\/\u2212<\/sup> CD8 + CGRP\uff08n = 9\uff09\uff1bb: \u6bcf\u7ec4n = 4\uff1bc: \u5b8c\u6574 + \u6eb6\u5242\u5bf9\u7167\uff08n = 15\uff09\uff0c\u6d88\u9664 + CGRP\uff08n = 11\uff09\uff1bd: \u6eb6\u5242\u5bf9\u7167\uff08n = 13\uff09\uff0cBIBN\u9884\u9632\uff08n = 16\uff09\uff0cBIBN\u6cbb\u7597\uff08n = 18\uff09\uff1be: \u6eb6\u5242\u5bf9\u7167\uff08n = 10\uff09\uff0cBIBN\u9884\u9632\uff08n = 13\uff09\uff0cBIBN\u6cbb\u7597\uff08n = 16\uff09\u3002<\/p>\n

\u5b9e\u9a8c\u72ec\u7acb\u91cd\u590d\u4e09\u6b21\uff0c\u7ed3\u679c\u76f8\u4f3c\u3002P\u503c\u901a\u8fc7\u5355\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u914d\u5408\u4e8b\u540eBonferroni\u6821\u6b63\uff08a,e\uff09\u3001\u53cc\u4fa7\u672a\u914d\u5bf9\u5b66\u751ft\u68c0\u9a8c\uff08b\uff09\u6216\u53cc\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u914d\u5408\u4e8b\u540eBonferroni\u6821\u6b63\uff08c,d\uff09\u786e\u5b9a\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u56fe 5 | CGRP\u51cf\u5f31RAMP1+ <\/sup>CD8+ <\/sup>T\u7ec6\u80de\u7684\u6297\u80bf\u7624\u514d\u75ab\u529b<\/strong><\/p>\n

a\u2013c, \u4eceRamp1WT<\/sup>\uff08CD45.1+<\/sup>\uff09\u6216Ramp1\u2212\/\u2212<\/sup>\uff08CD45.2+<\/sup>\uff09\u5c0f\u9f20\u4e2d\u7eaf\u5316\u5206\u79bb\u813e\u810fCD8+ <\/sup>T\u7ec6\u80de\uff0c\u4f53\u5916\u6269\u589e\u5e76\u523a\u6fc0\uff08CD3\u548cCD28 + IL-2\uff09\u3002\u5c06\u6fc0\u6d3b\u7684Ramp1\u2212\/\u2212<\/sup>\u6216Ramp1WT<\/sup> CD8+ <\/sup>T\u7ec6\u80de\u6216Ramp1\u2212\/\u2212<\/sup>\u4e0eRamp1WT<\/sup> CD8+ <\/sup>T\u7ec6\u80de1:1\u6df7\u5408\u7269\uff08\u6bcf\u53ea2.5 \u00d7 106<\/sup>\u7ec6\u80de\uff09\u901a\u8fc7\u9759\u8109\u79fb\u690d\u81f3\u516b\u5468\u5927\u7684\u96cc\u6027Rag1\u2212\/\u2212<\/sup>\u5c0f\u9f20\u3002\u79fb\u690d\u540e\u4e00\u5468\uff0c\u5c0f\u9f20\u88ab\u63a5\u79cdB16F10-mCherry-OVA\u7ec6\u80de\uff085 \u00d7 105<\/sup>\u7ec6\u80de\uff0ci.d.\uff09\u3002\u5728B16F10\u63a5\u79cd\u540e\u5341\u5929\uff0c\u6211\u4eec\u89c2\u5bdf\u5230\u5728Ramp1WT<\/sup>\u79fb\u690d\u7684\u5c0f\u9f20\u4e2d\u80bf\u7624\u751f\u957f\u66f4\u5feb\uff08a\uff09\u3002\u80bf\u7624\u5185\u7684Ramp1\u2212\/\u2212<\/sup>\uff08CD45.2+<\/sup>\uff09\u548cRamp1WT<\/sup>\uff08CD45.1+<\/sup>\uff09CD8+ <\/sup>T\u7ec6\u80de\u88ab\u6d41\u5f0f\u7ec6\u80de\u672f\u7eaf\u5316\u3001\u514d\u75ab\u8868\u578b\u9274\u5b9a\uff08b\uff09\u5e76\u8fdb\u884cRNA\u6d4b\u5e8f\uff08c\uff09\u3002Ramp1\u2212\/\u2212<\/sup> CD8+ <\/sup>T\u7ec6\u80de\u663e\u793a\u51fa\u8f83\u4f4e\u6bd4\u4f8b\u7684PD-1+<\/sup> LAG3+ <\/sup>TIM3+ <\/sup>CD8+ <\/sup>T\u7ec6\u80de\uff08b\uff09\uff0c\u4ee5\u53ca\u51cf\u5c11\u7684\u8017\u7aed\u6807\u8bb0\u7269\u8f6c\u5f55\u672c\u8868\u8fbe\uff08c\uff09\u3002<\/p>\n

d, \u5229\u7528\u764c\u75c7\u57fa\u56e0\u7ec4\u56fe\u8c31\uff08TCGA\uff09\u6570\u636e\u7684\u4f53\u5185\u5206\u679040\u7528\u6765\u76f8\u5173\u8054459\u540d\u9ed1\u8272\u7d20\u7624\u60a3\u8005\u7684\u751f\u5b58\u7387\u4e0eRAMP1\u8868\u8fbe\u6c34\u5e73\uff08\u539f\u59cb\u6d3b\u68c0\u5927\u89c4\u6a21RNA\u6d4b\u5e8f\uff09\u3002\u4e0eRAMP1\u8868\u8fbe\u4f4e\u7684\u60a3\u8005\u76f8\u6bd4\uff0c\u66f4\u9ad8\u7684RAMP1\u6c34\u5e73\u4e0e\u60a3\u8005\u751f\u5b58\u7387\u4e0b\u964d\u76f8\u5173\u3002<\/p>\n

e, \u4eba\u7c7b\u9ed1\u8272\u7d20\u7624\u7684\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u7684\u4f53\u5185\u5206\u6790\u63ed\u793a\uff0c\u4e0eRamp1\u9634\u6027CD8+ <\/sup>T\u7ec6\u80de\u76f8\u6bd4\uff0c\u80bf\u7624\u5185RAMP1\u8868\u8fbe\u7684CD8+ <\/sup>T\u7ec6\u80de\u5f3a\u70c8\u8fc7\u8868\u8fbe\u6570\u4e2a\u514d\u75ab\u68c0\u67e5\u70b9\u53d7\u4f53\uff08PD-1\uff08\u4e5f\u79f0\u4e3aPDCD1\uff09\u3001TIM3\u3001LAG3\u3001CTLA4\uff09\u3002<\/p>\n

\u6570\u636e\u4ee5\u5e73\u5747\u503c \u00b1 s.e.m.\uff08a\uff09\u3001\u5761\u5ea6\u56fe\uff08b\uff09\u3001\u70ed\u56fe\u663e\u793a\u6807\u51c6\u5316\u57fa\u56e0\u8868\u8fbe\uff08log10<\/sub>\uff08103<\/sup> \u00d7 TPM\uff09\uff09\uff08c\uff09\u3001Mantel\u2013Cox\u56de\u5f52\uff08d\uff09\u6216\u5c0f\u63d0\u7434\u56fe\uff08e\uff09\u663e\u793a\u3002<\/p>\n

\u5b9e\u9a8c\u72ec\u7acb\u91cd\u590d\u4e24\u6b21\uff08a,b\uff09\u5f97\u5230\u76f8\u4f3c\u7ed3\u679c\u3002\u6d4b\u5e8f\u5b9e\u9a8c\u672a\u91cd\u590d\uff08c\uff09\u3002<\/p>\n

P\u503c\u901a\u8fc7\u53cc\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u914d\u5408\u4e8b\u540eBonferroni\u6821\u6b63\uff08a\uff09\u3001\u53cc\u4fa7\u672a\u914d\u5bf9\u5b66\u751ft\u68c0\u9a8c\uff08b\uff09\u6216Mantel\u2013Cox\u56de\u5f52\uff08d\uff09\u786e\u5b9a\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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OncoLab\u516c\u4f17\u53f7\u5df2\u589e\u52a0\u671f\u520a\u67e5\u8be2\u529f\u80fd<\/span><\/strong>\uff0cSCI\u671f\u520a<\/strong><\/span>\u53ef\u67e5\u8be2\u671f\u520a\u540d\u79f0\u7f29\u5199\u3001ISSN\u520a\u53f7\u3001\u8fd1\u4e94\u5e74\u5f71\u54cd\u56e0\u5b50<\/strong>\u3001\u6700\u65b0WOS\u7684JCR\u5206\u533a<\/strong>\u3001\u6700\u65b0\u4e2d\u79d1\u9662\u5206\u533a<\/strong>\u4ee5\u53ca\u4e2d\u79d1\u9662\u671f\u520a\u9884\u8b66\u4fe1\u606f<\/strong>\uff1b<\/p>\n

\u4e2d\u6587\u671f\u520a<\/span><\/strong>\u53ef\u68c0\u7d22CSCD\u6838\u5fc3<\/strong>\u3001\u5317\u5927\u4e2d\u6587\u6838\u5fc3<\/strong>\u3001\u79d1\u6280\u6838\u5fc3<\/strong>\u6536\u5f55\u60c5\u51b5\uff1b<\/p>\n

\u5728\u516c\u4f17\u53f7\u5bf9\u8bdd\u6846\u53d1\u9001\u6742\u5fd7\u540d\u79f0<\/span><\/strong>\u5373\u53ef\u5feb\u901f\u68c0\u7d22\uff0c\u652f\u6301\u6a21\u7cca\u68c0\u7d22\uff0c\u6b22\u8fce\u4f7f\u7528\uff01<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n


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\u2014 THE  END \u2014<\/span><\/section>\n
\u6765\u6e90 | Nature<\/span><\/section>\n
\u7b5b\u9009\u6574\u7406 | \u738b\u5764<\/span><\/span><\/span><\/section>\n
\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u53ef\u8f6c\u5230\u6587\u7ae0\u9875\u9762
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\u516c\u4f17\u53f7\u53d1\u900120221102\u53ef\u83b7\u53d6\u6587\u732e\u5168\u6587<\/span><\/span><\/span><\/section>\n


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\u514d\u75ab\u5b66\u62fe\u9057\u7cfb\u5217<\/a><\/span><\/p>\n

\u80bf\u7624\u514d\u75ab\u5b66\u7cfb\u5217<\/span><\/a><\/p>\n

ACIR\u6587\u732e\u901f\u9012<\/span><\/a><\/p>\n

SCI\u8bba\u6587\u4f5c\u56fe<\/span><\/a><\/p>\n

SCI\u8bba\u6587\u5199\u4f5c\u53ca\u6295\u7a3f<\/span><\/a><\/p>\n

\u751f\u4fe1\u5165\u95e8\u5b66\u4e60\u7b14\u8bb0<\/span><\/a><\/p>\n

R\u8bed\u8a00\u57fa\u7840\u4e0e\u5355\u7ec6\u80de\u5206\u6790\u5b9e\u6218\u89c6\u9891\u8bfe\u7a0b<\/span><\/a><\/p>\n

\u751f\u7269\u5b66\u5b9e\u9a8c\u6280\u672f\u53ca\u5e38\u7528\u8f6f\u4ef6<\/span><\/a><\/p>\n

RCIM\u4eea\u5668\u7b80\u4ecb\u53ca\u64cd\u4f5c\u89c6\u9891<\/a><\/span><\/p>\n

\u5e38\u7528\u7535\u8111\u8f6f\u4ef6\u4f7f\u7528\u6559\u7a0b<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u52a0\u5165\u8bfb\u8005\u4ea4\u6d41\u7fa4~<\/span><\/p>\n

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\u70b9\u4eae\u8d5e\u4e0e\u5728\u770b <\/span><\/p>\n

\u8ba9\u66f4\u591a\u4eba\u770b\u5230<\/span><\/p>\n


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\u672c\u7bc7\u6587\u7ae0\u6765\u6e90\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7:OncoLab<\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

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