{"id":5499,"date":"2024-04-20T23:03:57","date_gmt":"2024-04-20T15:03:57","guid":{"rendered":"http:\/\/dh.oncolab.cn\/index.php\/2024\/04\/20\/cell%ef%bc%9a%e5%be%ae%e7%94%9f%e7%89%a9%e4%bb%a3%e8%b0%a2%e4%ba%a7%e7%89%a9%e9%80%9a%e8%bf%87%e8%b0%83%e8%8a%82t%e7%bb%86%e8%83%9e%e5%b9%b2%e6%80%a7%e5%a2%9e%e5%bc%ba%e6%b3%9b%e7%99%8c%e5%85%8d\/"},"modified":"2024-04-20T23:03:57","modified_gmt":"2024-04-20T15:03:57","slug":"cell%ef%bc%9a%e5%be%ae%e7%94%9f%e7%89%a9%e4%bb%a3%e8%b0%a2%e4%ba%a7%e7%89%a9%e9%80%9a%e8%bf%87%e8%b0%83%e8%8a%82t%e7%bb%86%e8%83%9e%e5%b9%b2%e6%80%a7%e5%a2%9e%e5%bc%ba%e6%b3%9b%e7%99%8c%e5%85%8d","status":"publish","type":"post","link":"http:\/\/dh.oncolab.cn\/index.php\/2024\/04\/20\/cell%ef%bc%9a%e5%be%ae%e7%94%9f%e7%89%a9%e4%bb%a3%e8%b0%a2%e4%ba%a7%e7%89%a9%e9%80%9a%e8%bf%87%e8%b0%83%e8%8a%82t%e7%bb%86%e8%83%9e%e5%b9%b2%e6%80%a7%e5%a2%9e%e5%bc%ba%e6%b3%9b%e7%99%8c%e5%85%8d\/","title":{"rendered":"Cell\uff1a\u5fae\u751f\u7269\u4ee3\u8c22\u4ea7\u7269\u901a\u8fc7\u8c03\u8282T\u7ec6\u80de\u5e72\u6027\u589e\u5f3a\u6cdb\u764c\u514d\u75ab\u6cbb\u7597\u6548\u679c"},"content":{"rendered":"
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\u6742\u5fd7\u540d\u79f0<\/strong>\uff1aCell<\/span><\/p>\n

\u53d1\u8868\u65e5\u671f<\/strong>\uff1a2024.03.11<\/p>\n

DOI<\/strong>\uff1a10.1016\/j.cell.2024.02.022.<\/p>\n

PubMed<\/strong>\uff1ahttps:\/\/pubmed.ncbi.nlm.nih.gov\/38490195\/<\/p>\n

\u5f71\u54cd\u56e0\u5b50<\/strong>\uff1a64.5<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u5185\u5bb9\u7b80\u4ecb<\/strong><\/p>\n<\/section>\n

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\u4eba\u7c7b\u764c\u75c7\u4e2d\u7684\u514d\u75ab\u68c0\u67e5\u70b9\u963b\u65ad\uff08ICB\uff09\u53cd\u5e94\u4e0e\u80a0\u9053\u83cc\u7fa4\u5bc6\u5207\u76f8\u5173\u3002\u6211\u4eec\u7684\u7814\u7a76\u53d1\u73b0\uff0c\u5171\u751f\u4e73\u6746\u83ccLactobacillus johnsonii\u7684\u4e30\u5ea6\u4e0eICB\u7684\u54cd\u5e94\u6027\u6b63\u76f8\u5173\u3002\u8865\u5145Lactobacillus johnsonii\u6216\u8272\u6c28\u9178\u884d\u751f\u7684\u4ee3\u8c22\u4ea7\u7269\u5432\u54da-3-\u4e19\u9178\uff08IPA\uff09\u53ef\u4ee5\u589e\u5f3aCD8+ T\u7ec6\u80de\u4ecb\u5bfc\u7684\u03b1PD-1\u514d\u75ab\u6cbb\u7597\u7684\u6548\u679c\u3002\u4ece\u673a\u5236\u4e0a\u8bb2\uff0cLactobacillus johnsonii\u4e0eClostridium sporogenes\u534f\u540c\u4f5c\u7528\u4ea7\u751fIPA\u3002IPA\u8c03\u8282CD8+ T\u7ec6\u80de\u7684\u5e72\u6027\u7a0b\u5e8f\uff0c\u5e76\u901a\u8fc7\u589e\u52a0Tcf7\u8d85\u589e\u5f3a\u5b50\u533a\u57df\u7684H3K27\u4e59\u9170\u5316\uff0c\u4fc3\u8fdb\u524d\u4f53\u8017\u7aedCD8+ T\u7ec6\u80de\uff08Tpex\uff09\u7684\u751f\u6210\u3002IPA\u5728\u6cdb\u764c\u75c7\u6c34\u5e73\u4e0a\u6539\u5584ICB\u7684\u54cd\u5e94\u6027\uff0c\u5305\u62ec\u9ed1\u8272\u7d20\u7624\u3001\u4e73\u817a\u764c\u548c\u7ed3\u76f4\u80a0\u764c\u3002\u603b\u7684\u6765\u8bf4\uff0c\u6211\u4eec\u7684\u53d1\u73b0\u8bc6\u522b\u4e86\u4e00\u6761\u5fae\u751f\u7269\u4ee3\u8c22\u4ea7\u7269-\u514d\u75ab\u8c03\u8282\u9014\u5f84\uff0c\u5e76\u63d0\u51fa\u4e86\u4e00\u79cd\u6f5c\u5728\u7684\u57fa\u4e8e\u5fae\u751f\u7269\u7684\u8f85\u52a9\u65b9\u6cd5\u6765\u63d0\u9ad8\u514d\u75ab\u6cbb\u7597\u7684\u54cd\u5e94\u6027\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u521b\u65b0\u6027\u53ca\u7814\u7a76\u610f\u4e49<\/strong><\/p>\n<\/section>\n

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\u672c\u7814\u7a76\u63d0\u51fa\u4e86\u4e00\u79cd\u5168\u65b0\u7684\u514d\u75ab\u6cbb\u7597\u7b56\u7565\uff0c\u5373\u901a\u8fc7\u8c03\u8282T\u7ec6\u80de\u5e72\u6027\u6765\u589e\u5f3a\u6cdb\u764c\u514d\u75ab\u6cbb\u7597\u7684\u6548\u679c\u3002\u7814\u7a76\u663e\u793a\uff0c\u80a0\u9053\u5e38\u4f4f\u83cc\u4e73\u6746\u83ccLactobacillus johnsonii\u7684\u4e30\u5bcc\u5ea6\u4e0e\u514d\u75ab\u68c0\u67e5\u70b9\u963b\u65ad\uff08ICB\uff09\u6cbb\u7597\u7684\u54cd\u5e94\u6027\u6b63\u76f8\u5173\uff0c\u4e14\u4e73\u6746\u83cc\u80fd\u4e0e\u68ad\u83ccClostridium sporogenes\u5171\u540c\u4f5c\u7528\uff0c\u751f\u6210\u8272\u6c28\u9178\u884d\u751f\u7684\u4ee3\u8c22\u4ea7\u7269\u5432\u54da-3-\u4e19\u9178\uff08IPA\uff09\u3002IPA\u80fd\u591f\u8c03\u8282CD8+ T\u7ec6\u80de\u7684\u5e72\u6027\u7f16\u7a0b\uff0c\u5e76\u901a\u8fc7\u589e\u52a0Tcf7\u57fa\u56e0\u8d85\u7ea7\u589e\u5f3a\u5b50\u533a\u57df\u7684H3K27\u4e59\u9170\u5316\uff0c\u4fc3\u8fdb\u524d\u4f53\u8017\u7aedCD8+ T\u7ec6\u80de\uff08Tpex\uff09\u7684\u5f62\u6210\uff0c\u4ece\u800c\u5728\u5305\u62ec\u9ed1\u8272\u7d20\u7624\u3001\u4e73\u817a\u764c\u548c\u7ed3\u76f4\u80a0\u764c\u5728\u5185\u7684\u591a\u79cd\u764c\u75c7\u4e2d\u63d0\u9ad8ICB\u7684\u53cd\u5e94\u6027\u3002<\/p>\n

\u8fd9\u9879\u7814\u7a76\u7684\u521b\u65b0\u6027\u5728\u4e8e\u63ed\u793a\u4e86\u5fae\u751f\u7269\u4ee3\u8c22\u4ea7\u7269\u5728\u8c03\u63a7\u514d\u75ab\u53cd\u5e94\u4e2d\u7684\u5173\u952e\u89d2\u8272\uff0c\u7279\u522b\u662f\u5728\u901a\u8fc7\u8c03\u8282\u7279\u5b9a\u57fa\u56e0\u7684\u8868\u89c2\u9057\u4f20\u4fee\u9970\u6765\u5f71\u54cdT\u7ec6\u80de\u7684\u529f\u80fd\u3002\u6b64\u5916\uff0c\u7814\u7a76\u8fd8\u5c55\u793a\u4e86\u5fae\u751f\u7269\u4ee3\u8c22\u4ea7\u7269IPA\u4f5c\u4e3a\u4e00\u79cd\u6f5c\u5728\u7684\u514d\u75ab\u7597\u6cd5\u4f50\u5242\uff0c\u53ef\u4ee5\u5728\u4e0d\u540c\u7c7b\u578b\u7684\u764c\u75c7\u4e2d\u589e\u5f3aICB\u6cbb\u7597\u7684\u6548\u679c\u3002\u8fd9\u4e00\u53d1\u73b0\u4e0d\u4ec5\u4e3a\u80a0\u9053\u5fae\u751f\u7269\u4e0e\u5bbf\u4e3b\u514d\u75ab\u7cfb\u7edf\u7684\u76f8\u4e92\u4f5c\u7528\u63d0\u4f9b\u4e86\u65b0\u7684\u673a\u5236\u7406\u89e3\uff0c\u4e5f\u4e3a\u4e34\u5e8a\u4e0a\u6539\u5584\u514d\u75ab\u6cbb\u7597\u6548\u679c\u5f00\u8f9f\u4e86\u65b0\u7684\u7b56\u7565\uff0c\u5177\u6709\u91cd\u8981\u7684\u7814\u7a76\u610f\u4e49\u548c\u5e94\u7528\u524d\u666f\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6570\u636e\u56fe<\/strong><\/p>\n<\/section>\n

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\u56fe 1. L. johnsonii \u901a\u8fc7\u6fc0\u6d3b\u80bf\u7624\u6d78\u6da6\u7684CD8+ T\u7ec6\u80de\uff0c\u589e\u5f3a\u6297PD-1\u514d\u75ab\u7597\u6cd5\u7684\u6548\u679c<\/strong><\/p>\n

\uff08A\uff09\u793a\u610f\u56fe\u5c55\u793a\u4e86\u5728\u76ae\u4e0bMc38\u5f02\u79cd\u79fb\u690d\u7624\u6a21\u578b\u4e2d\u6297PD-1\u514d\u75ab\u7597\u6cd5\u7684\u8fc7\u7a0b\u3002<\/p>\n

\uff08B\uff09\u76ae\u4e0bMc38\u80bf\u7624\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\uff0c\u5bf9\u6bd4\u4e86\u5bf9\u6297PD-1\u514d\u75ab\u7597\u6cd5\u53cd\u5e94\u826f\u597d\uff08\u54cd\u5e94\u8005\uff09\u548c\u53cd\u5e94\u8f83\u5dee\uff08\u4f4e\u54cd\u5e94\u8005\uff09\u7684\u5c0f\u9f20\u3002\u6bcf\u7ec45\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08C\uff09\u793a\u610f\u56fe\u5c55\u793a\u4e86\u7caa\u4fbf\u83cc\u7fa4\u79fb\u690d\u7684\u8fc7\u7a0b\u3002<\/p>\n

\uff08D\uff09\u63a5\u53d7\u4e86\u6297\u751f\u7d20\u5904\u7406\u5e76\u79fb\u690d\u4e86\u54cd\u5e94\u8005\u83cc\u7fa4\uff08R-\u63a5\u53d7\u8005\uff09\u6216\u4f4e\u54cd\u5e94\u8005\u83cc\u7fa4\uff08PR-\u63a5\u53d7\u8005\uff09\u7684\u5c0f\u9f20\uff0c\u5176\u76ae\u4e0bMc38\u80bf\u7624\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002\u6bcf\u7ec46\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08E\uff09\u4e3b\u5750\u6807\u5206\u6790\uff08PCoA\uff09\u56fe\u8868\u5c55\u793a\u4e86\u5728\u64cd\u4f5c\u5206\u7c7b\u5355\u5143\uff08OTU\uff09\u6c34\u5e73\u4e0a\uff0c\u54cd\u5e94\u8005\u548c\u4f4e\u54cd\u5e94\u8005\u5c0f\u9f20\u7684\u7caa\u4fbf\u6837\u672c\u768416S rRNA\u6d4b\u5e8f\u7ed3\u679c\u3002\u6bcf\u7ec45\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08F\uff09\u5728\u5c5e\u6c34\u5e73\u4e0a\uff0c\u901a\u8fc716S rRNA\u5e8f\u5217\u5206\u6790\u5bf9\u54cd\u5e94\u8005\u548c\u4f4e\u54cd\u5e94\u8005\u5c0f\u9f20\u7684\u7caa\u4fbf\u6837\u672c\u8fdb\u884c\u7684\u7fa4\u843d\u6761\u5f62\u56fe\u5206\u6790\u3002\u6bcf\u7ec45\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08G\uff09\u5728\u79cd\u6c34\u5e73\u4e0a\uff0c\u5bf9\u54cd\u5e94\u8005\u548c\u4f4e\u54cd\u5e94\u8005\u5c0f\u9f20\u7684\u7caa\u4fbf\u6837\u672c\u8fdb\u884cWilcoxon\u79e9\u548c\u6d4b\u8bd5\u7684\u6761\u5f62\u56fe\u5206\u6790\u3002\u6bcf\u7ec45\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08H\uff09\u5728\u63a5\u53d7\u4e86IgG\u540c\u578b\u5bf9\u7167\u6297\u4f53\u3001\u6297PD-1\u6297\u4f53\u3001L. johnsonii\u5355\u72ec\u4f7f\u7528\u6216\u4e0e\u6297PD-1\u6297\u4f53\u8054\u5408\u4f7f\u7528\u7684\u5904\u7406\u540e\uff0c\u5c0f\u9f20\u76ae\u4e0bMc38\u80bf\u7624\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002\u6bcf\u7ec47\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08I \u548c J\uff09\u76ae\u4e0bMc38\u80bf\u7624\u4e2dCD8+ T\u7ec6\u80de\u7684\u9891\u7387\u3002\uff08I\uff09\u5c55\u793a\u4e86\u4ee3\u8868\u6027\u7684\u6d41\u5f0f\u7ec6\u80de\u672f\u56fe\uff0c\uff08J\uff09\u5c55\u793a\u4e86\u7efc\u5408\u6570\u636e\u3002\u6bcf\u7ec47\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08B\uff09\u3001\uff08D\uff09\u548c\uff08H\uff09\u8868\u793a\u7684\u662f\u901a\u8fc7\u4e24\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u5e76\u7528Sidak\u7684\u591a\u91cd\u6bd4\u8f83\u6821\u6b63\u5f97\u51fa\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\uff08G\uff09\u8868\u793a\u7684\u662f\u901a\u8fc7Wilcoxon\u79e9\u548c\u6d4b\u8bd5\u5f97\u51fa\u7684\u5747\u503c \u00b1 \u6807\u51c6\u5dee\uff0c\u6ca1\u6709\u8fdb\u884c\u591a\u91cd\u6d4b\u8bd5\u3002\uff08J\uff09\u8868\u793a\u7684\u662f\u901a\u8fc7\u5355\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u5e76\u7528Sidak\u7684\u591a\u91cd\u6bd4\u8f83\u6821\u6b63\u5f97\u51fa\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\u8fd9\u4e9b\u6570\u636e\u4ee3\u8868\u4e86\u4e09\u6b21\u72ec\u7acb\u5b9e\u9a8c\u7684\u7ed3\u679c\u300216S rRNA\u662f\u630716S\u6838\u7cd6\u4f53RNA\uff1bL. j\u4ee3\u8868L. johnsonii\u3002*p < 0.05, **p < 0.01\u3002\u53e6\u89c1\u8865\u5145\u56fe1\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u56fe 2. L. johnsonii \u901a\u8fc7\u8272\u6c28\u9178\u4ee3\u8c22\u63d0\u5347\u514d\u75ab\u68c0\u67e5\u70b9\u963b\u65ad(ICB)\u7597\u6cd5\u7684\u53cd\u5e94\u6027<\/strong><\/p>\n

\uff08A \u548c B\uff09\uff08A\uff09\u5c55\u793a\u4e86\u5904\u7406\u4e0d\u540c\u6761\u4ef6\u4e0b\u7684\u5c0f\u9f20\u76ae\u4e0bMc38\u80bf\u7624\u7684\u4ee3\u8868\u6027\u56fe\u7247\uff0c\uff08B\uff09\u5219\u663e\u793a\u4e86\u8fd9\u4e9b\u80bf\u7624\u7684\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002\u5904\u7406\u6761\u4ef6\u5305\u62ec\u4f7f\u7528IgG\u5bf9\u7167\u6297\u4f53\u3001\u6297PD-1\u6297\u4f53\uff08aPD-1\uff09\u3001\u8054\u5408\u6d3bL. johnsonii\u7684\u6297PD-1\u6297\u4f53\uff08L. j + aPD-1\uff09\u3001MRS\u57f9\u517b\u57fa\uff08MRS + aPD-1\uff09\u3001L. johnsonii\u6761\u4ef6\u57f9\u517b\u57fa\uff08Lj.CM + aPD-1\uff09\u3001\u70ed\u706d\u6d3bL. johnsonii\uff08\u52a0\u70ed + aPD-1\uff09\u4ee5\u53ca\u8d85\u58f0\u6ce2\u5904\u7406\u7684L. johnsonii\uff08\u8d85\u58f0 + aPD-1\uff09\u3002\u6bcf\u7ec47\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08C\uff09\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u663e\u793a\u4e86\u63a5\u53d7\u6297PD-1\u4e0e\u540c\u65f6\u63a5\u53d7L. johnsonii\u548c\u6297PD-1\u5904\u7406\u7684\u5c0f\u9f20\u7684\u8840\u6d46\u4ee3\u8c22\u7269\uff0c\u8fd9\u4e9b\u4ee3\u8c22\u7269\u7531\u6db2\u76f8\u8272\u8c31-\u4e32\u8054\u8d28\u8c31\uff08LC-MS\/MS\uff09\u9274\u5b9a\u3002\u6bcf\u7ec46\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08D\uff09\u901a\u8fc7\u4eac\u90fd\u57fa\u56e0\u4e0e\u57fa\u56e0\u7ec4\u767e\u79d1\u5168\u4e66\uff08KEGG\uff09\u9014\u5f84\u5206\u6790\u5bf9\u6bd4\u4e86\u63a5\u53d7\u6297PD-1\u4e0e\u540c\u65f6\u63a5\u53d7L. johnsonii\u548c\u6297PD-1\u5904\u7406\u7684\u5c0f\u9f20\u4e2d\u4e0d\u540c\u5bcc\u96c6\u7684\u8840\u6d46\u4ee3\u8c22\u7269\u3002\u6bcf\u7ec46\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08E\uff09\u5728\u63a5\u53d7\u8272\u6c28\u9178\u7f3a\u4e4f\uff08Trpneg\uff09\u996e\u98df\u7684\u6761\u4ef6\u4e0b\uff0c\u7814\u7a76\u4e86\u5c0f\u9f20\u63a5\u53d7\u6297PD-1\u6297\u4f53\u5355\u72ec\u4f7f\u7528\u4e0e\u8054\u5408L. johnsonii\u4f7f\u7528\u65f6\u76ae\u4e0bMc38\u80bf\u7624\u7684\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002\u6bcf\u7ec47\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08F \u548c G\uff09\u76ae\u4e0bMc38\u80bf\u7624\u4e2dCD8+ T\u7ec6\u80de\u7684\u9891\u7387\u3002\uff08F\uff09\u5c55\u793a\u4e86\u4ee3\u8868\u6027\u7684\u6d41\u5f0f\u7ec6\u80de\u672f\u56fe\uff0c\uff08G\uff09\u5219\u663e\u793a\u4e86\u7efc\u5408\u6570\u636e\u3002\u6bcf\u7ec47\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08B\uff09\u548c\uff08E\uff09\u7684\u6570\u636e\u8868\u793a\u901a\u8fc7\u4e24\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u5e76\u7528Sidak\u7684\u591a\u91cd\u6bd4\u8f83\u6821\u6b63\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\uff08G\uff09\u7684\u6570\u636e\u5219\u901a\u8fc7\u672a\u914d\u5bf9t\u68c0\u9a8c\u5206\u6790\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\u8fd9\u4e9b\u6570\u636e\u4ee3\u8868\u4e86\u4e09\u6b21\u72ec\u7acb\u5b9e\u9a8c\u7684\u7ed3\u679c\u3002L. j\u4ee3\u8868L. johnsonii\uff1bMRS\u4ee3\u8868de Man, Rogosa\u548cSharpe\u57f9\u517b\u57fa\uff1bCM\u4ee3\u8868\u6761\u4ef6\u57f9\u517b\u57fa\uff1bTrpneg diet\u4ee3\u8868\u8272\u6c28\u9178\u7f3a\u4e4f\u996e\u98df\u3002ns\u8868\u793a\u65e0\u663e\u8457\u6027\uff0c*p < 0.05, **p < 0.01\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
\n
<\/section>\n<\/section>\n
\n

\u56fe 3. \u6765\u81eaL. johnsonii\u7684IPA\u901a\u8fc7CD8+ T\u7ec6\u80de\u63d0\u5347ICB\u7597\u6cd5\u7684\u53cd\u5e94\u6027<\/strong><\/p>\n

\uff08A\uff09\u70ed\u56fe\u663e\u793a\u4e86\u63a5\u53d7\u6297PD-1\u7597\u6cd5\u5355\u72ec\u6216\u7ed3\u5408L. johnsonii\u6cbb\u7597\u7684\u5c0f\u9f20\u4e2d\uff0c\u4e0e\u8272\u6c28\u9178\u76f8\u5173\u7684\u8840\u6d46\u4ee3\u8c22\u7269\u7684\u5bcc\u96c6\u60c5\u51b5\u3002\u6bcf\u7ec46\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08B\uff09\u6db2\u76f8\u8272\u8c31-\u4e32\u8054\u8d28\u8c31\uff08LC-MS\/MS\uff09\u7528\u4e8e\u786e\u5b9a\u63a5\u53d7\u54cd\u5e94\u8005\u548c\u4f4e\u54cd\u5e94\u8005\u83cc\u7fa4\u79fb\u690d\u7684\u5c0f\u9f20\u8840\u6d46\u4e2dIPA\u7684\u542b\u91cf\u3002\u6bcf\u7ec46\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08C\uff09\u6db2\u76f8\u8272\u8c31-\u4e32\u8054\u8d28\u8c31\uff08LC-MS\/MS\uff09\u7528\u4e8e\u786e\u5b9a\u5728\u63a5\u53d7\u8272\u6c28\u9178\u7f3a\u4e4f\uff08Trpneg\uff09\u996e\u98df\u548c\u6297PD-1\u7597\u6cd5\u5355\u72ec\u6216\u7ed3\u5408L. johnsonii\u6cbb\u7597\u7684\u5c0f\u9f20\u8840\u6d46\u4e2dIPA\u7684\u542b\u91cf\u3002\u6bcf\u7ec47\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08D\uff09\u5728\u63a5\u53d7\u6297PD-1\u7597\u6cd5\u5355\u72ec\u3001\u7ed3\u5408L. johnsonii\uff08L. j + aPD-1\uff09\u6216\u7ed3\u5408IPA\uff08IPA + aPD-1\uff09\u7684\u6cbb\u7597\u540e\uff0c\u5c0f\u9f20\u76ae\u4e0bMc38\u80bf\u7624\u7684\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002\u6bcf\u7ec47\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08E \u548c F\uff09\u76ae\u4e0bMc38\u80bf\u7624\u4e2dCD8+ T\u7ec6\u80de\u7684\u9891\u7387\u3002\uff08E\uff09\u5c55\u793a\u4e86\u4ee3\u8868\u6027\u7684\u6d41\u5f0f\u7ec6\u80de\u672f\u56fe\uff0c\uff08F\uff09\u5c55\u793a\u4e86\u7efc\u5408\u6570\u636e\u3002\u6bcf\u7ec47\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08G\uff09\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790\u76ae\u4e0bMc38\u80bf\u7624\u4e2dIFN-\u03b3+ CD8+ T\u7ec6\u80de\u7684\u9891\u7387\u3002\u6bcf\u7ec47\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08H\uff09\u5728Rag1\u2212\/\u2212\u5c0f\u9f20\u4e2d\uff0c\u63a5\u53d7\u6297PD-1\u7597\u6cd5\u5355\u72ec\u6216\u7ed3\u5408L. johnsonii\u6216IPA\u7684\u6cbb\u7597\u540e\uff0c\u76ae\u4e0bMc38\u80bf\u7624\u7684\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002\u6bcf\u7ec46\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08I\uff09\u5728Rag1\u2212\/\u2212\u5c0f\u9f20\u4e2d\uff0c\u63a5\u53d7IgG\u540c\u578b\u5bf9\u7167\u6297\u4f53\u3001\u6297PD-1\u3001IPA\u5355\u72ec\u6216IPA\u548c\u6297PD-1\u8054\u5408\u6cbb\u7597\u540e\uff0c\u76ae\u4e0bMc38\u80bf\u7624\u7684\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002\u6bcf\u7ec45\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08J\uff09\u63a5\u53d7\u6297PD-1\u7597\u6cd5\u5355\u72ec\u6216\u7ed3\u5408L. johnsonii\u6216IPA\u7684\u6cbb\u7597\uff0c\u4ee5\u53ca\u540c\u65f6\u63a5\u53d7\u6297CD8\u548c\u6297PD-1\u6297\u4f53\u4ee5\u53caL. johnsonii\u6216IPA\u7684\u6cbb\u7597\u7684\u5c0f\u9f20\uff0c\u76ae\u4e0bMc38\u80bf\u7624\u7684\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002\u6bcf\u7ec47\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08K\uff09\u793a\u610f\u56fe\u663e\u793a\u4e86\u5f02\u4f53\u7ec6\u80de\u8f6c\u79fb\uff08ACT\uff09\u7684\u8fc7\u7a0b\u3002\u4f9b\u4f53\u5c0f\u9f20\u63a5\u53d7\u6297PD-1\u7597\u6cd5\u5355\u72ec\u6216\u7ed3\u5408IPA\u7684\u6cbb\u7597\u3002\u6765\u81ea\u4f9b\u4f53\u5c0f\u9f20\u7684B\u7ec6\u80de\u6216\uff08\u53ca\uff09CD8+ T\u7ec6\u80de\u6839\u636e\u663e\u793a\u8f6c\u79fb\u5230\u63a5\u53d7\u8005\u5c0f\u9f20\uff08Rag1\u2212\/\u2212\uff09\u4e2d\u3002<\/p>\n

\uff08L\uff09\u5728\u63a5\u53d7\u8005\u5c0f\u9f20\u4e2d\uff0c\u76ae\u4e0bMc38\u80bf\u7624\u7684\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002\u6bcf\u7ec45\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08D\uff09\u3001\uff08H\uff09-\uff08J\uff09\u548c\uff08L\uff09\u7684\u6570\u636e\u8868\u793a\u901a\u8fc7\u4e24\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u5e76\u7528Sidak\u7684\u591a\u91cd\u6bd4\u8f83\u6821\u6b63\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\uff08F\uff09\u548c\uff08G\uff09\u7684\u6570\u636e\u901a\u8fc7\u5355\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u5e76\u7528Sidak\u7684\u591a\u91cd\u6bd4\u8f83\u6821\u6b63\u5f97\u51fa\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\u8fd9\u4e9b\u6570\u636e\u4ee3\u8868\u4e86\u4e09\u6b21\u72ec\u7acb\u5b9e\u9a8c\u7684\u7ed3\u679c\u3002L. j\u4ee3\u8868L. johnsonii\uff1bIPA\u4ee3\u8868\u5432\u54da-3-\u4e19\u9178\uff1bFMT\u4ee3\u8868\u7caa\u4fbf\u83cc\u7fa4\u79fb\u690d\uff1bTrpneg diet\u4ee3\u8868\u8272\u6c28\u9178\u7f3a\u4e4f\u996e\u98df\uff1bACT\u4ee3\u8868\u5f02\u4f53\u7ec6\u80de\u8f6c\u79fb\uff1bWT\u4ee3\u8868\u91ce\u751f\u578b\u3002ns\u8868\u793a\u65e0\u663e\u8457\u6027\uff0c*p < 0.05, **p < 0.01, ****p < 0.0001\u3002\u53e6\u89c1\u8865\u5145\u56feS2\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
\n
<\/section>\n<\/section>\n
\n

\u56fe 4. IPA\u901a\u8fc7\u4fc3\u8fdbTpex\u7ec6\u80de\u7684\u6d3b\u6027\u6765\u7ef4\u6301CD8+ T\u7ec6\u80de\u7684\u53cd\u5e94<\/strong><\/p>\n

\uff08A\uff09\u793a\u610f\u56fe\u5c55\u793a\u4e86\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\uff08scRNA-seq\uff09\u3001\u5355\u7ec6\u80deTCR\u6d4b\u5e8f\uff08scTCR-seq\uff09\u548c\u9ad8\u901a\u91cf\u6d4b\u5e8f\u7528\u4e8e\u9776\u5411\u5f00\u653e\u67d3\u8272\u8d28\u7684\u5355\u7ec6\u80de\u5206\u6790\uff08scATAC-seq\uff09\u7684\u6d41\u7a0b\u3002<\/p>\n

\uff08B\uff09\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u548cUMAP\u805a\u7c7b\u5206\u6790\uff0c\u7528\u4e8e\u4ece\u63a5\u53d7\u6297PD-1\u548c\u540c\u65f6\u63a5\u53d7\u6297PD-1\u53caIPA\u5904\u7406\u7684\u5c0f\u9f20\u4e2d\u5206\u7c7b\u540e\u7684CD8+ T\u7ec6\u80de\u3002<\/p>\n

\uff08C\uff09\u5806\u53e0\u6761\u5f62\u56fe\u663e\u793a\u4e86\u5728\u63a5\u53d7\u6297PD-1\uff08WT, n = 2\uff09\u548c\u540c\u65f6\u63a5\u53d7\u6297PD-1\u53caIPA\uff08IPA, n = 2\uff09\u5904\u7406\u7684\u5c0f\u9f20\u4e2d\uff0c\u6bcf\u79cd\u8bc6\u522b\u51fa\u7684\u7ec6\u80de\u7c7b\u578b\u5728CD8+ T\u7ec6\u80de\u4e2d\u7684\u767e\u5206\u6bd4\u3002<\/p>\n

\uff08D\uff09\u793a\u610f\u56fe\u5c55\u793a\u4e86\u57fa\u4e8eVISION\u7684\u5206\u6790\u6d41\u7a0b\u3002<\/p>\n

\uff08E\uff09\u4ee3\u8868\u6027\u7684UMAP\u56fe\u663e\u793a\u4e86\u5728WT\u548cIPA\u5c0f\u9f20\u4e2d\uff0c\u524d\u4f53\u8017\u7aedCD8+ T\u7ec6\u80de\uff08\u4ee5\u84dd\u8272\u7a81\u51fa\u663e\u793a\uff09\u7684\u5206\u5e03\u3002<\/p>\n

\uff08F\uff09\u6563\u70b9\u56fe\u663e\u793a\u4e86\u5728WT\uff08n = 2\uff09\u548cIPA\uff08n = 2\uff09\u5c0f\u9f20\u4e2d\uff0c\u6bcf\u4e2a\u7ec6\u80de\u7fa4\u96c6\u7684GO_ALPHA_BETA_T_CELL_DIFFERENTIATION\u57fa\u56e0\u96c6\u7684\u5e73\u5747\u7b7e\u540d\u5f97\u5206\u3002<\/p>\n

\uff08G\uff09\u5c0f\u63d0\u7434\u56fe\uff08\u5de6\uff09\u548cECDF\u56fe\uff08\u53f3\uff09\u5c55\u793a\u4e86WT\u548cIPA\u5c0f\u9f20\u4e2d\u524d\u4f53\u8017\u7aedCD8+ T\u7ec6\u80de\u7fa4\u96c6\u7684VISION\u5bcc\u96c6\u5f97\u5206\uff0c\u9488\u5bf9GO_ALPHA_BETA_T_CELL_DIFFERENTIATION\u57fa\u56e0\u96c6\u3002<\/p>\n

\uff08H\uff09\u793a\u610f\u56fe\u5c55\u793a\u4e86CD8+ T\u7ec6\u80de\u7684\u4f53\u5916\u57f9\u517b\u8fc7\u7a0b\u3002<\/p>\n

\uff08I\uff09\u4ee3\u8868\u6027\u76f4\u65b9\u56fe\uff08\u5de6\uff09\u548c\u7ed3\u5408\u7684\u5e73\u5747\u8367\u5149\u5f3a\u5ea6\uff08MFI\uff09\uff08\u53f3\uff09\u5c55\u793a\u4e86\u7528\u6d41\u5f0f\u7ec6\u80de\u672f\u6d4b\u91cf\u7684\u7ecfDMSO\u6216IPA\u5904\u7406\u7684PD-1+ CD8+ T\u7ec6\u80de\u4e2dTCF-1\u7684\u8868\u8fbe\u3002CD8+ T\u7ec6\u80de\u4ece\u5c0f\u9f20\u7684\u6dcb\u5df4\u7ed3\u6216\u813e\u810f\u4e2d\u7eaf\u5316\u3002<\/p>\n

\uff08J\uff09Tcf7\u2212\/\u2212\u5c0f\u9f20\u53ca\u5176WT\u540c\u7a9d\u63a7\u5236\u5c0f\u9f20\u7684\u80bf\u7624\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\uff0c\u4e24\u79cd\u5c0f\u9f20\u5747\u63a5\u53d7\u4e86\u6297PD-1\u5355\u72ec\u6216\u4e0eIPA\u7ed3\u5408\u7684\u5904\u7406\u3002\u6bcf\u7ec45\u53ea\u5c0f\u9f20\u3002<\/p>\n

\uff08K\uff09\u901a\u8fc7monocle 2\u5728\u4e8c\u7ef4\u72b6\u6001\u7a7a\u95f4\u5b9a\u4e49\u7684CD8+ T\u7ec6\u80de\u7684\u5047\u65f6\u95f4\u8f68\u8ff9\uff0c\u989c\u8272\u6df1\u6d45\u8868\u793a\u5047\u65f6\u95f4\uff08\u5de6\uff09\uff0c\u989c\u8272\u4ee3\u8868\u4e0d\u540c\u7684\u8bc6\u522b\u51fa\u7684T\u7ec6\u80de\u4e9a\u7fa4\uff08\u53f3\uff09\u3002<\/p>\n

\uff08L\uff09\u4e92\u8fde\u548c\u5f26\u56fe\u663e\u793a\u4e86\u5728WT\u548c\u7ecfIPA\u5904\u7406\u7684\u6837\u672c\u4e2dCD8+ T\u7ec6\u80de\u4e9a\u7fa4\u5171\u4eab\u7684TCRs\uff08\u9876\u90e8\uff09\uff0c\u4ee5\u53ca\u5728WT\u548c\u7ecfIPA\u5904\u7406\u7684\u6837\u672c\u4e2d\u524d\u4f53\u8017\u7aedCD8+ T\u7ec6\u80de\u4e9a\u7fa4\u4e0e\u6548\u5e94CD8+ T\u7ec6\u80de\u4e9a\u7fa4\u4e4b\u95f4\u5171\u4eab\u76f8\u540c\u7684CDR3\uff08\u914d\u5bf9\u7684TCR\u03b1\u548cTCR\u03b2\u94fe\uff09\u6c28\u57fa\u9178\u5e8f\u5217\u7684\u6570\u91cf\u7684\u97e6\u6069\u56fe\uff08\u5e95\u90e8\uff09\u3002<\/p>\n

\uff08M\uff09WT\u548c\u7ecfIPA\u5904\u7406\u7684\u6837\u672c\u4e2d\uff0c\u8de8CD8+ T\u7ec6\u80de\u4e9a\u7fa4\u7684\u514b\u9686\u5bc6\u5ea6\u56fe\u3002UMAPs\u4e3a\u6807\u8bb0\u7684T\u7ec6\u80de\u4e9a\u7fa4\u7c07\u8fdb\u884c\u4e86\u989c\u8272\u7f16\u7801\u3002<\/p>\n

\uff08G\uff09\u7684\u6570\u636e\u901a\u8fc7Wilcoxon\u79e9\u548c\u6d4b\u8bd5\u5206\u6790VISION\u5bcc\u96c6\u5f97\u5206\u3002\uff08I\uff09\u7684\u6570\u636e\u8868\u793a\u901a\u8fc7\u672a\u914d\u5bf9t\u68c0\u9a8c\u5206\u6790\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\uff08J\uff09\u7684\u6570\u636e\u901a\u8fc7\u4e24\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u5e76<\/p>\n

\u7528Sidak\u7684\u591a\u91cd\u6bd4\u8f83\u6821\u6b63\u5f97\u51fa\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\u8fd9\u4e9b\u6570\u636e\u4ee3\u8868\u4e86\u4e09\u6b21\u72ec\u7acb\u5b9e\u9a8c\u6216\u751f\u7269\u91cd\u590d\u3002ECDF\u4ee3\u8868\u7ecf\u9a8c\u7d2f\u79ef\u5206\u5e03\u51fd\u6570\uff1bIPA\u4ee3\u8868\u5432\u54da-3-\u4e19\u9178\uff1bUMAP\u4ee3\u8868\u5747\u5300\u6d41\u5f62\u903c\u8fd1\u548c\u6295\u5f71\uff1bWT\u4ee3\u8868\u91ce\u751f\u578b\u3002ns\u8868\u793a\u65e0\u663e\u8457\u6027\u5dee\u5f02\uff0c*p < 0.05, **p < 0.01\u3002\u53e6\u89c1\u56feS3\u548cS4\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\n

\u56fe 5. IPA\u901a\u8fc7\u8c03\u6574Tcf7\u57fa\u56e0\u8d85\u7ea7\u589e\u5f3a\u5b50\u533a\u57df\u7684H3K27\u4e59\u9170\u5316\u6fc0\u6d3bTpex\u7ec6\u80de<\/strong><\/p>\n

\uff08A\uff09\u5355\u7ec6\u80deATAC\u6d4b\u5e8f\uff08scATAC-seq\uff09\u548cUMAP\u805a\u7c7b\u5206\u6790\uff0c\u7528\u4e8e\u4ece\u63a5\u53d7\u6297PD-1\uff08WT\uff09\u548c\u540c\u65f6\u63a5\u53d7\u6297PD-1\u53caIPA\uff08IPA\uff09\u5904\u7406\u7684\u5c0f\u9f20\u4e2d\u5206\u7c7b\u540e\u7684CD8+ T\u7ec6\u80de\u3002<\/p>\n

\uff08B\uff09\u5806\u53e0\u6761\u5f62\u56fe\u663e\u793a\u4e86\u5728WT\uff08n = 2\uff09\u548cIPA\uff08n = 2\uff09\u5904\u7406\u7684\u5c0f\u9f20\u4e2d\uff0c\u6bcf\u79cd\u8bc6\u522b\u51fa\u7684\u7ec6\u80de\u7c7b\u578b\u5728CD8+ T\u7ec6\u80de\u4e2d\u7684\u767e\u5206\u6bd4\u3002<\/p>\n

\uff08C\uff09\u70ed\u56fe\u663e\u793a\u4e86\u5728WT\uff08n = 2\uff09\u548cIPA\uff08n = 2\uff09\u5c0f\u9f20\u4e2d\uff0c\u524d\u4f53\u8017\u7aedCD8+ T\u7ec6\u80de\u7fa4\u96c6\u7684\u4e0d\u540c\u67d3\u8272\u8d28\u53ef\u8bbf\u95ee\u6027\u5cf0\u4f4d\u7f6e\u3002<\/p>\n

\uff08D\uff09\u5c0f\u63d0\u7434\u56fe\uff08\u5de6\uff09\u548cECDF\u56fe\uff08\u53f3\uff09\u5c55\u793a\u4e86WT\u548cIPA\u5c0f\u9f20\u4e2d\u524d\u4f53\u8017\u7aedCD8+ T\u7ec6\u80de\u7fa4\u96c6\u7684\u201cGO_POSITIVE_REGULATION_OF_HISTONE_ACETYLATION\u201d\u57fa\u56e0\u96c6\u7684VISION\u5bcc\u96c6\u5f97\u5206\u3002<\/p>\n

\uff08E\uff09\u5c0f\u63d0\u7434\u56fe\uff08\u5de6\uff09\u548cECDF\u56fe\uff08\u53f3\uff09\u5c55\u793a\u4e86WT\u548cIPA\u5c0f\u9f20\u4e2d\u524d\u4f53\u8017\u7aedCD8+ T\u7ec6\u80de\u7fa4\u96c6\u7684\u201cGO_HISTONE_H3_ACETYLATION\u201d\u57fa\u56e0\u96c6\u7684VISION\u5bcc\u96c6\u5f97\u5206\u3002<\/p>\n

\uff08F\uff09\u901a\u8fc7Western blot\u5206\u6790\uff0c\u68c0\u6d4b\u4e86\u4e0eIPA\u5171\u57f9\u517b\u6216\u672a\u5171\u57f9\u517b\u7684CD8+ T\u7ec6\u80de\u4e2dTCF-1\u3001H2AK5ac\u3001H2BK5ac\u3001H3K27ac\u3001H4K20me1\u3001\u7ec4\u86cb\u767dH2A\u3001H2B\u3001H3\u548cH4\u7684\u8868\u8fbe\uff08\u6d53\u5ea6\u5206\u522b\u4e3a0, 5, 500 mM\uff09\u3002<\/p>\n

\uff08G\uff09ChIP-qPCR\u65b9\u6cd5\u68c0\u6d4b\u4e86\u4e0eIPA\u5171\u57f9\u517b\u6216\u672a\u5171\u57f9\u517b\u7684CD8+ T\u7ec6\u80de\u4e2dTcf7\u57fa\u56e0\u8d85\u7ea7\u589e\u5f3a\u5b50\u4f4d\u70b9\u7684H3K27\u4e59\u9170\u5316\u60c5\u51b5\u3002\u6bcf\u7ec43\u4e2a\u751f\u7269\u91cd\u590d\u3002<\/p>\n

\uff08H\uff09CUT&RUN-qPCR\u65b9\u6cd5\u68c0\u6d4b\u4e86\u4e0eIPA\u5171\u57f9\u517b\u6216\u672a\u5171\u57f9\u517b\u7684CD8+ T\u7ec6\u80de\u4e2dTcf7\u57fa\u56e0\u8d85\u7ea7\u589e\u5f3a\u5b50\u4f4d\u70b9\u7684H3K27\u4e59\u9170\u5316\u60c5\u51b5\u3002\u6bcf\u7ec44\u4e2a\u751f\u7269\u91cd\u590d\u3002<\/p>\n

\uff08I\uff09CUT&Tag\u6d4b\u5e8f\u5206\u6790\u4e86\u4e0eIPA\u5171\u57f9\u517b\u6216\u672a\u5171\u57f9\u517b\u7684CD8+ T\u7ec6\u80de\u4e2d\u7684H3K27\u4e59\u9170\u5316\u60c5\u51b5\uff0c\u4e59\u9170\u5316\u5cf0\u503c\u5728\u4e0a\u5c42\u9762\u677f\u663e\u793a\u3002\u6bcf\u7ec46\u4e2a\u751f\u7269\u91cd\u590d\u3002<\/p>\n

\uff08J\uff09\u901a\u8fc7\u5355\u7ec6\u80deATAC\u6d4b\u5e8f\uff08scATAC-seq\uff0c\u6bcf\u7ec42\u4e2a\u91cd\u590d\uff09\u3001H3K27ac\u6d4b\u5e8f\uff08\u6bcf\u7ec46\u4e2a\u91cd\u590d\uff09\u6216\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\uff08scRNA-seq\uff0c\u6bcf\u7ec42\u4e2a\u91cd\u590d\uff09\u5206\u6790\uff0c\u5728\u6307\u5b9a\u6761\u4ef6\u4e0bTcf7\u57fa\u56e0\u4f4d\u70b9\u7684\u57fa\u56e0\u7ec4\u7ed3\u5408\u6a21\u5f0f\u7684\u4ee3\u8868\u6027\u63d2\u56fe\u3002\u9ed1\u8272\u865a\u7ebf\u8868\u793a\u5f3a\u5ea6\u6709\u5dee\u5f02\u7684\u5cf0\u3002Tcf7\u57fa\u56e0\u533a\u57df\u4ee5\u7c89\u8272\u7a81\u51fa\u663e\u793a\uff0c\u8d85\u7ea7\u589e\u5f3a\u5b50\u533a\u57df\u4ee5\u84dd\u8272\u7a81\u51fa\u663e\u793a\u3002<\/p>\n

\uff08D\uff09\u548c\uff08E\uff09\u7684\u6570\u636e\u901a\u8fc7Wilcoxon\u79e9\u548c\u6d4b\u8bd5\u5206\u6790VISION\u5bcc\u96c6\u5f97\u5206\u3002\uff08G\uff09\u7684\u6570\u636e\u8868\u793a\u901a\u8fc7\u53cc\u5c3e\u6bd4\u7387\u914d\u5bf9t\u68c0\u9a8c\u5206\u6790\u7684\u5747\u503c \u00b1 \u6807\u51c6\u5dee\u3002\uff08H\uff09\u7684\u6570\u636e\u901a\u8fc7\u53cc\u5c3e\u6bd4\u7387\u914d\u5bf9t\u68c0\u9a8c\u5206\u6790CUT&RUN-qPCR\u3002\u8fd9\u4e9b\u6570\u636e\u4ee3\u8868\u4e863\u81f36\u6b21\u72ec\u7acb\u5b9e\u9a8c\u6216\u751f\u7269\u91cd\u590d\u3002IPA\u4ee3\u8868\u5432\u54da-3-\u4e19\u9178\uff1bSE\u4ee3\u8868\u8d85\u7ea7\u589e\u5f3a\u5b50\u3002***p < 0.001\u3002\u53e6\u89c1\u56feS5\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u56fe 6. L. johnsonii \u4e0e C. sporogenes \u5171\u540c\u4f5c\u7528\u4ea7\u751fIPA<\/strong><\/p>\n

\uff08A\uff09\u901a\u8fc7\u6db2\u76f8\u8272\u8c31-\u4e32\u8054\u8d28\u8c31\uff08LC-MS\/MS\uff09\u68c0\u6d4bMRS\u57f9\u517b\u57fa\u548cL. johnsonii\u6761\u4ef6\u57f9\u517b\u57fa\uff08Lj. CM\uff09\u4e0a\u6e05\u6db2\u4e2d\u7684IPA\u3002n = 6\u6b21\u72ec\u7acb\u5b9e\u9a8c\u3002<\/p>\n

\uff08B\uff09\u901a\u8fc7LC-MS\/MS\u68c0\u6d4bMRS\u57f9\u517b\u57fa\u548cLj.CM\u4e0a\u6e05\u6db2\u4e2d\u7684\u5432\u54da-3-\u4e73\u9178\uff08ILA\uff09\u3002n = 6\u6b21\u72ec\u7acb\u5b9e\u9a8c\u3002<\/p>\n

\uff08C\uff09\u76ae\u4e0bMc38\u80bf\u7624\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\uff0c\u5c0f\u9f20\u63a5\u53d7\u6297PD-1\u6cbb\u7597\uff0c\u6297PD-1\u8054\u5408IPA\u6216ILA\u6cbb\u7597\uff0c\u6216\u6297PD-1\u8054\u5408ILA\u548c\u6297\u751f\u7d20\uff08Abx\uff09\u6cbb\u7597\u3002n = 6\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08D\uff09\u901a\u8fc7LC-MS\/MS\u68c0\u6d4b\u63a5\u53d7\u4e0a\u8ff0\u6307\u5b9a\u6cbb\u7597\u7684\u5c0f\u9f20\u8840\u6d46\u4e2d\u7684IPA\u3002n = 6\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08E\uff09\u901a\u8fc7\u8179\u8154\u6ce8\u5c04\uff0c\u5c0f\u9f20\u63a5\u53d7\u6297PD-1\u6216\u6297PD-1\u8054\u5408ILA\u6216IPA\u6cbb\u7597\u540e\u7684\u76ae\u4e0bMc38\u80bf\u7624\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002n = 6\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08F\uff09\u901a\u8fc7LC-MS\/MS\u68c0\u6d4b\u63a5\u53d7\u4e0a\u8ff0\u6307\u5b9a\u6cbb\u7597\u7684\u5c0f\u9f20\u8840\u6d46\u4e2d\u7684IPA\u3002n = 6\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08G\uff09\u793a\u610f\u56fe\u5c55\u793a\u4e86\u5728\u65e0\u83cc\u5c0f\u9f20\u4e0a\u8fdb\u884c\u6297PD-1\u514d\u75ab\u6cbb\u7597\u7684\u8fc7\u7a0b\u3002<\/p>\n

\uff08H\uff09\u5728\u5355\u72ec\u5b9a\u690dL. johnsonii\u6216\uff08\u548c\uff09C. sporogenes\u7684\u65e0\u83cc\u5c0f\u9f20\u4e2d\uff0c\u63a5\u53d7\u6297PD-1\u6cbb\u7597\u540e\u7684\u76ae\u4e0bMc38\u80bf\u7624\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002n = 7\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08I\uff09\u901a\u8fc7LC-MS\/MS\u68c0\u6d4b\u63a5\u53d7\u4e0a\u8ff0\u6307\u5b9a\u6cbb\u7597\u7684\u65e0\u83cc\u5c0f\u9f20\u8840\u6d46\u4e2d\u7684IPA\u3002n = 7\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08J\uff09\u793a\u610f\u56fe\u5c55\u793a\u4e86\u86cb\u767d\u8d28BLAST\u5206\u6790\u548c\u5728\u5927\u80a0\u6746\u83cc\u4e2d\u8fc7\u8868\u8fbeldhA\u57fa\u56e0\u7684\u8fc7\u7a0b\u3002<\/p>\n

\uff08K\uff09\u5c0f\u9f20\u63a5\u53d7\u6297PD-1\uff0c\u6216\u6297PD-1\u8054\u5408\u5927\u80a0\u6746\u83cc\u6216\u5de5\u7a0b\u5927\u80a0\u6746\u83cc\uff08E. c-ldhA\uff09\u6cbb\u7597\u540e\u7684\u76ae\u4e0bMc38\u80bf\u7624\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002n = 7\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08L\uff09\u901a\u8fc7LC-MS\/MS\u68c0\u6d4b\u63a5\u53d7\u4e0a\u8ff0\u6307\u5b9a\u6cbb\u7597\u7684\u5c0f\u9f20\u8840\u6d46\u4e2d\u7684IPA\u3002n = 7\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08C\uff09\u3001\uff08E\uff09\u3001\uff08H\uff09\u548c\uff08K\uff09\u7684\u6570\u636e\u8868\u793a\u901a\u8fc7\u4e24\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u5e76\u7528Sidak\u7684\u591a\u91cd\u6bd4\u8f83\u6821\u6b63\u5f97\u51fa\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\u8fd9\u4e9b\u6570\u636e\u4ee3\u8868\u4e86\u4e09\u6b21\u72ec\u7acb\u5b9e\u9a8c\u3002ILA\u4ee3\u8868\u5432\u54da-3-\u4e73\u9178\uff1bIPA\u4ee3\u8868\u5432\u54da-3-\u4e19\u9178\uff1bAbx\u4ee3\u8868\u6297\u751f\u7d20\uff1bL. j\u4ee3\u8868L. johnsonii\uff1bC. s\u4ee3\u8868C. sporogenes\u3002ns\u8868\u793a\u65e0\u663e\u8457\u6027\uff0c*p < 0.05, ****p < 0.0001\u3002\u53e6\u89c1\u56feS6\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u56fe 7. IPA\u5728\u591a\u79cd\u764c\u75c7\u548c\u7ed3\u76f4\u80a0\u764c\u6765\u6e90\u7684\u7c7b\u5668\u5b98\u4f53\u4e2d\u63d0\u9ad8ICB\u7597\u6cd5\u7684\u53cd\u5e94\u6027<\/strong><\/p>\n

\uff08A\uff09\u63a5\u53d7IgG\u540c\u578b\u5bf9\u7167\u3001IPA\u3001\u6297PD-1\u6216\u540c\u65f6\u63a5\u53d7IPA\u548c\u6297PD-1\u6cbb\u7597\u7684\u5c0f\u9f20\uff0c\u5176\u53ef\u79fb\u690d4T1\u4e73\u817a\u80bf\u7624\u7684\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002n = 7\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08B\uff09\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790\u4ece4T1\u4e73\u817a\u80bf\u7624\u4e2d\u5206\u79bb\u7684CD8+ T\u7ec6\u80de\u4e2dTCF-1\u7684\u5e73\u5747\u8367\u5149\u5f3a\u5ea6\uff08MFI\uff09\u3002n = 7\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08C\uff09\u63a5\u53d7IgG\u540c\u578b\u5bf9\u7167\u3001IPA\u3001\u6297PD-1\u6216\u540c\u65f6\u63a5\u53d7IPA\u548c\u6297PD-1\u6cbb\u7597\u7684\u5c0f\u9f20\uff0c\u5176\u53ef\u79fb\u690dB16-F10\u9ed1\u8272\u7d20\u7624\u7684\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002n = 7\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08D\uff09\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790\u4eceB16-F10\u9ed1\u8272\u7d20\u7624\u4e2d\u5206\u79bb\u7684CD8+ T\u7ec6\u80de\u4e2dTCF-1\u7684\u5e73\u5747\u8367\u5149\u5f3a\u5ea6\uff08MFI\uff09\u3002n = 7\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08E\uff09\u63a5\u53d7\u6297PD-1\u6216\u4e0eIPA\u8054\u5408\u6cbb\u7597\u7684\u5c0f\u9f20\uff0c\u51764T1\u539f\u4f4d\u4e73\u817a\u80bf\u7624\u7684\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002n = 7\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08F\uff09\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790\u4ece4T1\u539f\u4f4d\u4e73\u817a\u80bf\u7624\u4e2d\u5206\u79bb\u7684CD8+ T\u7ec6\u80de\u4e2dTCF-1\u7684\u5e73\u5747\u8367\u5149\u5f3a\u5ea6\uff08MFI\uff09\u3002n = 7\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08G\uff09\u63a5\u53d7\u6297PD-1\u6216\u4e0eIPA\u8054\u5408\u6cbb\u7597\u7684MMTV-PyMT\u5c0f\u9f20\u7684\u751f\u5b58\u66f2\u7ebf\u3002n = 10\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08H\uff09\u63a5\u53d7\u6297PD-1\u6216\u4e0eIPA\u8054\u5408\u6cbb\u7597\u7684\u5c0f\u9f20\uff0c\u5176Mc38\u539f\u4f4d\u80bf\u7624\u7684\u4f53\u79ef\u53d8\u5316\uff08\u5355\u4f4d\uff1a\u7acb\u65b9\u6beb\u7c73\uff09\u3002n = 5\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08I\uff09\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790\u4eceMc38\u539f\u4f4d\u80bf\u7624\u4e2d\u5206\u79bb\u7684CD8+ T\u7ec6\u80de\u4e2dTCF-1\u7684\u5e73\u5747\u8367\u5149\u5f3a\u5ea6\uff08MFI\uff09\u3002n = 5\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08J\uff09\u4e24\u7ec4\u5c0f\u9f20\u4e2d\uff0c\u4e73\u817a\u764c\u3001\u9ed1\u8272\u7d20\u7624\u548c\u7ed3\u76f4\u80a0\u764c\u4e2dTCF-1+ CD8+\u7ec6\u80de\u4e0eDAPI+\u7ec6\u80de\u7684\u9891\u7387\u3002n = 6\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08K\uff09\u4ee3\u8868\u6027\u56fe\u50cf\u663e\u793a\u63a5\u53d7IPA\u6cbb\u7597\u4e0e\u5426\u7684\u5c0f\u9f20\u4e2d\u4e73\u817a\u764c\u3001\u9ed1\u8272\u7d20\u7624\u548c\u7ed3\u76f4\u80a0\u764c\u6837\u672c\u7684\u514d\u75ab\u8367\u5149\u67d3\u8272\uff0cCD8\uff08\u7ea2\u8272\uff09\u3001TCF-1\uff08\u7eff\u8272\uff09\u548cDAPI\uff08\u84dd\u8272\uff09\uff08\u6807\u5c3a\uff0c20\u6beb\u7c73\uff09\u3002n = 6\u53ea\u5c0f\u9f20\u6bcf\u7ec4\u3002<\/p>\n

\uff08L\uff09\u793a\u610f\u56fe\u5c55\u793a\u7a7a\u6c14-\u6db2\u4f53\u754c\u9762\u7ed3\u76f4\u80a0\u764cPDO\uff08\u60a3\u8005\u884d\u751f\u7684\u7c7b\u5668\u5b98\u4f53\uff09\u7684\u5236\u5907\u8fc7\u7a0b\u3002<\/p>\n

\uff08M\uff09\u65b0\u9c9c\u4eba\u7c7b\u7ed3\u76f4\u80a0\u764c\u6d3b\u68c0\u7684H&E\u67d3\u8272\uff08\u4e0a\u56fe\uff0c\u6807\u5c3a\uff0c200\u6beb\u7c73\uff09\u4ee5\u53ca\u6cbb\u7597\u540ePDO\u7684H&E\u67d3\u8272\uff08\u4e0b\u56fe\uff0c\u6807\u5c3a\uff0c200\u6beb\u7c73\uff09\u3002n = 5\u4f4d\u60a3\u8005\u3002<\/p>\n

\uff08N\uff09\u7a7a\u6c14-\u6db2\u4f53\u754c\u9762PDO\u5728\u63a5\u53d7IPA\u6cbb\u7597\u4e0e\u5426\u7684\u6761\u4ef6\u4e0b\uff0cPRF1\u3001GZMB\u548cIFNG\u8868\u8fbe\u7684qPCR\u5206\u6790\u3002n = 3\u4f4d\u60a3\u8005\u3002<\/p>\n

\uff08A\uff09\u3001\uff08C\uff09\u548c\uff08E\uff09\u7684\u6570\u636e\u8868\u793a\u901a\u8fc7\u4e24\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u5e76\u7528Sidak\u7684\u591a\u91cd\u6bd4\u8f83\u6821\u6b63\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\uff08B\uff09\u548c\uff08D\uff09\u7684\u6570\u636e\u901a\u8fc7\u5355\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u5e76\u7528Sidak\u7684\u591a\u91cd\u6bd4\u8f83\u6821\u6b63\u5f97\u51fa\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\uff08F\uff09\u3001\uff08I\uff09\u548c\uff08J\uff09\u7684\u6570\u636e\u901a\u8fc7\u672a\u914d\u5bf9t\u68c0\u9a8c\u5f97\u51fa\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\uff08G\uff09\u7684\u751f\u5b58\u66f2\u7ebf\u901a\u8fc7\u5bf9\u6570\u79e9\u68c0\u9a8c\u5206\u6790\u3002\uff08H\uff09\u7684\u6570\u636e\u901a\u8fc7\u672a\u914d\u5bf9t\u68c0\u9a8c\u5e76\u5e94\u7528Welch\u7684\u6821\u6b63\u5f97\u51fa\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\uff08N\uff09\u7684\u6570\u636e\u8868\u793a\u901a\u8fc7\u53cc\u5c3e\u6bd4\u7387\u914d\u5bf9t\u68c0\u9a8c\u7684\u5747\u503c \u00b1 \u6807\u51c6\u5dee\u3002\u8fd9\u4e9b\u6570\u636e\u4ee3\u8868\u4e86\u4e09\u6b21\u72ec\u7acb\u5b9e\u9a8c\u3002IPA\u4ee3\u8868\u5432\u54da-3-\u4e19\u9178\uff1bCRC\u4ee3\u8868\u7ed3\u76f4\u80a0\u764c\u3002ns\u8868\u793a\u65e0\u663e\u8457\u6027\u5dee\u5f02\uff0c*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001\u3002\u53e6\u89c1\u56feS7\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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OncoLab\u516c\u4f17\u53f7\u5df2\u589e\u52a0\u671f\u520a\u67e5\u8be2\u529f\u80fd<\/span><\/strong>\uff0cSCI\u671f\u520a<\/strong><\/span>\u53ef\u67e5\u8be2\u671f\u520a\u540d\u79f0\u7f29\u5199\u3001ISSN\u520a\u53f7\u3001\u8fd1\u4e94\u5e74\u5f71\u54cd\u56e0\u5b50<\/strong>\u3001\u6700\u65b0WOS\u7684JCR\u5206\u533a<\/strong>\u3001\u6700\u65b0\u4e2d\u79d1\u9662\u5206\u533a<\/strong>\u4ee5\u53ca\u4e2d\u79d1\u9662\u671f\u520a\u9884\u8b66\u4fe1\u606f<\/strong>\uff1b<\/p>\n

\u4e2d\u6587\u671f\u520a<\/span><\/strong>\u53ef\u68c0\u7d22CSCD\u6838\u5fc3<\/strong>\u3001\u5317\u5927\u4e2d\u6587\u6838\u5fc3<\/strong>\u3001\u79d1\u6280\u6838\u5fc3<\/strong>\u6536\u5f55\u60c5\u51b5\uff1b<\/p>\n

\u5728\u516c\u4f17\u53f7\u5bf9\u8bdd\u6846\u53d1\u9001\u6742\u5fd7\u540d\u79f0<\/span><\/strong>\u5373\u53ef\u5feb\u901f\u68c0\u7d22\uff0c\u652f\u6301\u6a21\u7cca\u68c0\u7d22\uff0c\u6b22\u8fce\u4f7f\u7528\uff01<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

\u2014 THE  END \u2014<\/span><\/section>\n
\u6765\u6e90 | Cell<\/span><\/section>\n
\u7b5b\u9009\u6574\u7406 | \u738b\u5764<\/span><\/span><\/span><\/section>\n
\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u53ef\u8f6c\u5230\u6587\u7ae0\u9875\u9762
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\u516c\u4f17\u53f7\u53d1\u90012024031113\u53ef\u83b7\u53d6\u6587\u732e\u5168\u6587<\/span><\/span><\/span><\/section>\n


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\u2589  \u5f80\u671f\u63a8\u9001\u5408\u8f91<\/strong><\/strong><\/section>\n
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\u514d\u75ab\u5b66\u62fe\u9057\u7cfb\u5217<\/a><\/span><\/p>\n

\u80bf\u7624\u514d\u75ab\u5b66\u7cfb\u5217<\/span><\/a><\/p>\n

ACIR\u6587\u732e\u901f\u9012<\/span><\/a><\/p>\n

SCI\u8bba\u6587\u4f5c\u56fe<\/span><\/a><\/p>\n

SCI\u8bba\u6587\u5199\u4f5c\u53ca\u6295\u7a3f<\/span><\/a><\/p>\n

\u751f\u4fe1\u5165\u95e8\u5b66\u4e60\u7b14\u8bb0<\/span><\/a><\/p>\n

R\u8bed\u8a00\u57fa\u7840\u4e0e\u5355\u7ec6\u80de\u5206\u6790\u5b9e\u6218\u89c6\u9891\u8bfe\u7a0b<\/span><\/a><\/p>\n

\u751f\u7269\u5b66\u5b9e\u9a8c\u6280\u672f\u53ca\u5e38\u7528\u8f6f\u4ef6<\/span><\/a><\/p>\n

RCIM\u4eea\u5668\u7b80\u4ecb\u53ca\u64cd\u4f5c\u89c6\u9891<\/a><\/span><\/p>\n

\u5e38\u7528\u7535\u8111\u8f6f\u4ef6\u4f7f\u7528\u6559\u7a0b<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u5173\u6ce8\u672c\u53f7~<\/span>
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\u52a0\u5165\u8bfb\u8005\u4ea4\u6d41\u7fa4~<\/span><\/p>\n

\uff08\u6dfb\u52a0\u8bf7\u5907\u6ce8\u5355\u4f4d\u59d3\u540d\uff09<\/span><\/p>\n


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\u52a0\u5165\u77e5\u8bc6\u661f\u7403~<\/span><\/p>\n


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<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n


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\u70b9\u4eae\u8d5e\u4e0e\u5728\u770b <\/span><\/p>\n

\u8ba9\u66f4\u591a\u4eba\u770b\u5230<\/span><\/p>\n

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\u672c\u7bc7\u6587\u7ae0\u6765\u6e90\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7:OncoLab<\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u6742\u5fd7\u540d\u79f0\uff1aCell \u53d1\u8868\u65e5\u671f\uff1a2024.03.11 DOI\uff1a10.1016\/j.cell.2024.02.022. PubMed\uff1ahttps:\/\/pubmed.ncbi.nlm.n…<\/p>\n","protected":false},"author":1,"featured_media":5490,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[57],"tags":[],"_links":{"self":[{"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/posts\/5499"}],"collection":[{"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/comments?post=5499"}],"version-history":[{"count":0,"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/posts\/5499\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/media\/5490"}],"wp:attachment":[{"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/media?parent=5499"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/categories?post=5499"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/dh.oncolab.cn\/index.php\/wp-json\/wp\/v2\/tags?post=5499"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}